Rhinal Cortex Slices
Rhinal Cortex Slices - Related Videos
03:18
Preparing Rhinal Cortex-Hippocampus Slices from a Rat Pup Brain
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2025
The video demonstrates a method for obtaining rhinal cortex-hippocampus slices from a rat pup's brain and culturing them in membranous inserts within a multi-well plate filled with media for further analysis.
10:05
A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures
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Cited by 5 •
2021
Here, we describe the preparation of rhinal cortex-hippocampus organotypic slices. Under a gradual and controlled deprivation of serum, these slices depict evolving epileptic-like events and can be considered an ex vivo model of epileptogenesis. This system represents an excellent tool for monitoring the dynamics of spontaneous activity, as well as for assessing the progression of neuroinflammatory features throughout the course of epileptogenesis.
03:11
Generation of Medial Thalamus-Anterior Cingulate Cortex Slices from Mouse Brains
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2025
The video demonstrates the preparation of medial thalamus-anterior cingulate cortex (MT-ACC) slices from mouse brains. A mouse's brain is dissected, cuts are made to expose the MT-ACC pathway, and slices are generated using a vibratome. The MT-ACC slices are then maintained in oxygenated artificial cerebrospinal fluid.
02:44
Recording of Local Field Potential in Mouse Hippocampal-Entorhinal Cortex Slices
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2025
The video demonstrates a procedure to record the local field potential (LFP) in mouse hippocampal-entorhinal cortex slices. Electrical stimulation is provided at the CA3 stratum radiatum of the hippocampus, which causes changes in the postsynaptic potential at the CA1 stratum pyramidale. The combined change in the membrane potential of CA1 neurons, termed the local field potential, is recorded.
09:45
Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex
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Cited by 36 •
2012
We describe procedures for preparation and electrophysiological recording from brain slices that maintain the dorsal-ventral axis of the medial entorhinal cortex (MEC). Because neural encoding of location follows a dorsal-ventral organization within the MEC, these procedures facilitate investigation of cellular mechanisms important for navigation and memory.
FAQs
Preserved neurons and synaptic connections allow investigators to examine how rhinal circuits process and transmit information without relying only on whole-brain observations. This makes it possible to relate electrical activity, neurotransmission, and synaptic plasticity to local circuit organization. The preparation is especially useful for connecting cellular events with mechanisms relevant to learning, memory, and communication with hippocampal networks.
Oxygenated artificial cerebrospinal fluid provides the maintenance environment required for the slice preparation during experiments. Under these conditions, researchers can study neural activity and synaptic function in preserved tissue rather than examining fixed or inactive sections. Maintaining the preparation in this medium is therefore essential for electrophysiological and pharmacological measurements of living circuit processes.
Pharmacological analysis allows researchers to alter neurotransmission or related circuit processes while monitoring the resulting neural responses. Comparing activity before and after manipulation helps identify how particular signaling processes contribute to synaptic function and plasticity. In Rhinal Cortex Slices, this controlled approach links molecular or cellular changes to information transfer within rhinal circuits and toward hippocampal networks.
A typical workflow begins by preparing thin sections from the brain’s rhinal regions, including the perirhinal and entorhinal cortices. The sections are then maintained in oxygenated artificial cerebrospinal fluid before investigators apply electrophysiological or pharmacological analyses. These experiments can measure electrical activity, neurotransmission, and synaptic plasticity while allowing controlled manipulation of cellular and circuit-level processes.
These preparations support measurements of electrical activity, neurotransmission, and synaptic plasticity. Together, the measurements show how neurons respond, how signals move through local synaptic connections, and how circuit function changes under experimental conditions. Such results can clarify relationships among neuronal excitability, cortical connectivity, and the processing of information relevant to learning and memory.
Researchers use this preparation when they need controlled access to rhinal circuits involved in learning and memory. It supports studies of cortical connectivity, neuronal excitability, and the transfer of sensory information to hippocampal networks. The same approach can also contribute to investigations of neurological disorders by revealing cellular and circuit-level changes under defined experimental conditions.