זיהוי של חלבונים קושרי מולקולה קטנים בסביבה נייד Native ידי תיוג Live- תא Photoaffinity

0:43

Preparation of Cultured Cells

1:23

Treatment of Cells and Photocrosslinking

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Attachment of Fluorescent Tag by Click Chemistry for Visualization of Labeled Proteins

5:48

Attachment of Biotin Tag by Click Chemistry for Affinity Purification of Labeled Proteins

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Results: Live-cell Photoaffinity Labeling Identifies a Major Itraconazole-binding Protein

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Conclusion