Primary Rat Imcd Cells
Primary Rat Imcd Cells - Related Videos
06:11
Culturing Primary Rat Inner Medullary Collecting Duct Cells
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Cited by 19 •
2013
Arginine-vasopressin (AVP) controls fine-tuning of body water homeostasis through facilitating water reabsorption by renal principal cells. Here, we present a protocol for the cultivation of primary rat inner medullary collecting duct cells suitable for the elucidation of molecular mechanisms underlying AVP-mediated water reabsorption.
07:06
Isolation and Primary Culture of Rat Hepatic Cells
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Cited by 145 •
2012
Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.
10:20
Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
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Cited by 133 •
2012
Isolating primary microglia from the cellular heterogeneity of the brain is essential to investigate their role in both physiological and pathological conditions. This protocol describes a mechanical isolation and mixed cell culture technique that provides high yield and high purity, viable primary microglial cells for in vitro study and downstream applications.
13:36
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy
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Cited by 12 •
2014
This protocol describes a method of live cell imaging using primary rat neonatal cardiomyocytes following lentiviral and adenoviral transduction using confocal spinning disk microscopy. This enables detailed observations of cellular processes in living cardiomyocytes.
08:55
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
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Cited by 17 •
2017
This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological process.
FAQs
Vasopressin initiates a signaling sequence in which cAMP activates protein kinase A. This pathway promotes aquaporin-2 trafficking, relocating water-channel proteins in a way that increases epithelial water permeability. The response makes these cultured cells useful for connecting hormone-triggered intracellular signaling with the collecting duct’s role in regulating renal water balance.
Aquaporin-2 trafficking provides a cellular mechanism linking vasopressin signaling to altered water movement. Rather than representing signaling as an isolated biochemical event, the model allows researchers to examine how pathway activation changes a transport-related property of the epithelium. This helps explain how cellular responses contribute to broader osmoregulation by the kidney.
The key sequence described for this model includes vasopressin, cAMP, protein kinase A, and aquaporin-2. Vasopressin activates cAMP signaling, cAMP activates protein kinase A, and protein kinase A promotes aquaporin-2 trafficking. Studying these linked steps helps researchers investigate how hormonal information is transmitted from a receptor-level stimulus to altered epithelial water permeability.
This comparison shows whether a hormonal signal produces a functional transport consequence rather than only a molecular response. In primary rat IMCD cells, vasopressin-associated signaling can be related to aquaporin-2 trafficking and increased water permeability. Such measurements support analysis of osmoregulation and help connect cell-level findings with renal function at the whole-organ scale.
A study can begin with isolated cells maintained in culture, followed by exposure to vasopressin or another condition relevant to renal regulation. Researchers then examine signaling or transport-related responses, including cAMP and protein kinase A activity, aquaporin-2 trafficking, or water permeability. This workflow preserves a direct link between an experimental stimulus and epithelial behavior.
The cultured-cell model provides a more focused setting for examining epithelial mechanisms that are difficult to separate in an intact kidney. Researchers can investigate hormone signaling, water and solute regulation, and disease-related changes at the cellular level. Findings can then be interpreted in relation to whole-organ renal function, while potential therapeutics can be evaluated in a relevant ex vivo system.