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Tissue Targeted Embryonic Chimeras
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Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation

Article DOI: 10.3791/1422-v • 18:58 min • September 11th, 2009
September 11th, 2009 •
1, 1
1Department of Biological Sciences, Smith College

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Zebrafish cell transplantation enables the combination of genetics and embryology to generate tissue specific chimeras. This video demonstrates gastrula staged cell transplantations that have allowed our lab to investigate the roles of astroglial populations and specific guidance cues during commissure formation in the forebrain.

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Tissue Targeted Embryonic Chimeras Zebrafish Gastrula Cell Transplantation Developmental Biology Cell Functions Protein Misexpression Forebrain Commissures Neuron-glial Cell Interactions Astroglial Populations Guidance Cues Projecting Axons Midline Crossing Zebrafish Embryos Ectopic Cell Placement Gene Misexpression Vital Dye Tracer Fluorescent Protein Expression
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