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Journal / Immunology and Infection / Isolering och flödescytometrisk analys av…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering och flödescytometrisk analys av gliom-infiltrera perifera mononukleära blodceller
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Isolering och flödescytometrisk analys av gliom-infiltrera perifera mononukleära blodceller

Article DOI: 10.3791/53676-v • 12:52 min • November 28th, 2015
November 28th, 2015 •
1,2, 1,2, 1,2
1Department of Neurosurgery, University of Michigan, 2Department of Cell and Developmental Biology, University of Michigan

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Presenteras här är en enkel metod för isolering och flödescytometrisk analys av gliom-infiltrerande perifera mononukleära blodkroppar som ger tidsberoende kvantitativa uppgifter om antal och aktiveringsstatus av immunceller som förs in i hjärnans tidiga tumörmikro.

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Immunologi gliom perifera mononukleära blodceller (PBMC) galektin-1 (gal-1) GL26-Cit-gal1i GL26-Cit-NT Gr-1 naturliga mördarceller (NK)
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