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An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

Article DOI: 10.3791/54040-v • 08:11 min • August 14th, 2016
August 14th, 2016 •
1,2,3, 1,2,3
1Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, 2Georgia Cancer Center, Augusta University, 3Charlie Norwood VA Medical Center

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An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.

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Orthotopic Mouse Model Spontaneous Breast Cancer Metastasis Primary Tumor Growth Breast Cancer Metastasis Kinetics Tumor-associated Genes Therapeutic Agents Efficacy Testing 4T1 Luciferase Tumor Cells Mammary Gland Labeling Electronic Hair Trimmer Metastasis Mechanism Mortality Of Breast Cancer Patients
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