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斑马鱼 RNASeq-based 基因表达数据的样品制备与分析

Article DOI: 10.3791/56187-v • 11:42 min • October 27th, 2017
October 27th, 2017 •
*1, *1, 1
1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine
* These authors contributed equally

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该协议提出了一种从斑马鱼胚胎, 幼虫, 或排序细胞的整体转录分析的方法。我们包括 RNA 的分离, RNASeq 数据的通路分析, 和 qRT pcr 验证基因表达的变化。

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遗传学 问题 128 斑马鱼 RNAseq 转录 RNA,斑马鱼 通路分析 基因本体术语 流排序 荧光活化细胞分类
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