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Préparation des échantillons et l’analyse des données sur l’Expression génique à base RNASeq de poisson-zèbre

Article DOI: 10.3791/56187-v • 11:42 min • October 27th, 2017
October 27th, 2017 •
*1, *1, 1
1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine
* These authors contributed equally

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Ce protocole présente une approche pour l’analyse du transcriptome ensemble des embryons de poisson-zèbre, larves, ou trier des cellules. Nous incluons l’isolement de l’ARN, la voie analyse des données RNASeq et qRT-PCR-based validation des modifications de l’expression génique.

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Numéro 128 poisson zèbre RNAseq génétique transcriptome ARN Danio rerio analyse de la voie conditions Gene Ontology flux tri Fluorescence cellulaire activé tri
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