Produzione efficiente e identificazione di CRISPR/Cas9-generato Gene Ko nel sistema modello Danio rerio
21,930 Views
•
11:27 min
•
August 28, 2018
DOI:
Summary
Automatically generated
L'editing genomico mirato nel sistema modello Danio rerio (pesce zebra) notevolmente è stata facilitata dall'emergere di approcci basati su CRISPR. Qui, descriviamo un protocollo semplificato, robusto per la generazione e l'identificazione degli alleli di assurdità CRISPR-derivato che incorpora l'analisi di mobilità dell'eteroduplex e identificazione delle mutazioni mediante sequenziamento di nuova generazione.
Video correlati
08:25
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
Video correlati
13847 Views
11:35
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Video correlati
12496 Views
08:27
Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
Video correlati
13443 Views
08:37
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
Video correlati
7560 Views
07:01
Manipulation of Gene Function in Mexican Cavefish
Video correlati
9091 Views
10:08
Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
Video correlati
2188 Views
05:55
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
Video correlati
1230 Views
09:38
Fluorescence Labeling to Visualize Low-Expressed Proteins in Zebrafish
Video correlati
517 Views
11:27
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
Video correlati
21.9K Views
11:35
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Video correlati
12.5K Views
Read Article
Cite this Article
Sorlien, E. L., Witucki, M. A., Ogas, J. Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio. J. Vis. Exp. (138), e56969, doi:10.3791/56969 (2018).
Copy