Metodo per ripiegamento efficiente e purificazione del chemocettore Ligand Binding Domain
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December 12, 2017
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Una procedura è presentata per il ripiegamento del dominio legante dCACHE periplasmico ligando di Campylobacter jejuni del chemocettore Tlp3 da corpi di inclusione e la purificazione per produrre quantità di milligrammo di proteina.
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Machuca, M. A., Roujeinikova, A. Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain. J. Vis. Exp. (130), e57092, doi:10.3791/57092 (2017).
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