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Isolierung von Erwachsenen Rückenmark Kerne für massiv parallele Single-Kern-RNA-Sequenzierung
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Isolierung von Erwachsenen Rückenmark Kerne für massiv parallele Single-Kern-RNA-Sequenzierung

Article DOI: 10.3791/58413-v • 06:38 min • October 12th, 2018
October 12th, 2018 •
1, 1, 2, 3,4, 3, 1
1Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program, National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development, National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility, Frederick National Laboratory

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Hier präsentieren wir ein Protokoll, um schnell qualitativ hochwertige Kerne aus dem frischen oder gefrorenen Gewebe für die nachgelagerten massiv parallelen RNA Sequenzierung zu isolieren. Wir enthalten Waschmittel-mechanische und hypotone-mechanische Gewebe Störung und Zelle Lyse Optionen, die für die Isolierung von Kernen verwendet werden können.

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Neurowissenschaften Ausgabe 140 Kerne RNA Sequenzierung SnRNA-Seq massiv parallelen Rückenmark Saccharose gradient
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