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小鼠耳蜗特定频率区域带状带突起的形态和功能评估
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Neuroscienze
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JoVE Journal Neuroscienze
Morphological and Functional Evaluation of Ribbon Synapses at Specific Frequency Regions of the Mouse Cochlea
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小鼠耳蜗特定频率区域带状带突起的形态和功能评估

DOI:
10.3791/59189-v

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09:54 min

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May 10, 2019

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, , , , , , , , ,
1Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital,Capital Medical University, 2Department of Otology,Shengjing Hospital of China Medical University

Capitoli

  • 00:04Titolo
  • 00:50Animal Selection
  • 02:12Cochlear Tissue Processing
  • 04:08Immunofluorescence Staining
  • 05:53Morphological Cochlear Ribbon Synapse Evaluation
  • 07:37Results: Representative Cochlear Frequency Localization and Cochlear Ribbon Analysis
  • 09:08Conclusion

Summary

Traduzione automatica

Traduzione automatica

本手稿描述了用于评估正常小鼠带状突触的形态特征和功能状态的实验协议。本模型也适用于噪声诱发和与年龄相关的耳蜗性突触性受限模型。讨论了以往小鼠研究的相关结果。

Tags

CochleaFrequency RegionSynapseNeuropathyHearing LossTinnitusHyperacusisAuditory Brainstem ResponseABRCochlea DissectionParaformaldehydeEDTAMicroscopy

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