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In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Article DOI: 10.3791/59831-v • 07:10 min • June 28th, 2019
June 28th, 2019 •
1, 1, 1, 1, 2, 2, 1,3, 1
1Physics of Synthetic Systems - E14, Physics-Department and ZNN, Technische Universität München, 2Departmant of Chemistry, Center for Integrated Protein Science (CIPSM), Technische Universität München, 3Nanosystems Initiative Munich

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Automatic Translation

Presented here are protocols for the creation of peptide-based small unilamellar vesicles capable of growth. To facilitate in vesiculo production of the membrane peptide, these vesicles are equipped with a transcription-translation system and the peptide-encoding plasmid.

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Vesiculo Synthesis Peptide Membrane Precursors Autonomous Vesicle Growth Film Re-hydration Reaction Compartments Peptides Protocol Simplicity Robustness Sensitive Samples Centrifugal Vacuum Concentrator ELP Solution Chloroform Methanol Mixture Vortex Spherical Glass Beads Round Bottom Flask Rotary Evaporator Aluminum Foil Desiccator
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