Immunostaining dei test di Drosophila a montaggio intero per l'analisi confocale 3D di grandi spermatociti
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08:37 min
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August 27, 2020
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Presentiamo qui un protocollo migliorato per la preparazione a montaggio intero e l'immunostaining dei tester Drosophila, adatto per la microscopia confocale e che consente anche un'etichettatura riproducibile e affidabile. Illustriamo questo protocollo attraverso la rappresentazione 3D e la quantificazione degli esperimenti di colocalizzazione sui grandi spermatociti S4-S5.
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Cite this Article
Raich, N., Contremoulins, V., Karess, R. E. Immunostaining of Whole-Mount Drosophila Testes for 3D Confocal Analysis of Large Spermatocytes. J. Vis. Exp. (162), e61061, doi:10.3791/61061 (2020).
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