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Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
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Biologia
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JoVE Journal Biologia
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

DOI:
10.3791/61390-v

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08:42 min

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July 03, 2020

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, , , , , , ,
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology, 2Department of Stomatology,Dalian Medical University, 3The 2nd Dental Center, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology

Capitoli

  • 00:04Introduction
  • 00:54Specimen Collection
  • 02:13Paraffin Section Preparation
  • 03:34TRAP Staining
  • 05:34Calcein and Alizarin Red Double Labeling
  • 06:55Results: Effects of Stat3 Deletion on Osteoclast Differentiation
  • 08:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes a canonical method to understand the critical genes controlling osteoclast activity in vivo. This method uses a transgenic mouse model and some canonical techniques to analyze skeletal phenotype.

Tags

Skeletal Phenotype AnalysisGenetically Engineered Mouse ModelHind LimbParaffin SectioningMicro-CT ScanningCalcein And Alizarin Red Double LabelingDecalcificationParaffin Embedding

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