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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Article DOI: 10.3791/63730-v • 06:43 min • May 3rd, 2022
May 3rd, 2022 •
1, 1, 1,2
1Department of Molecular Biophysics & Biochemistry, Yale University, 2Department of Physics, Yale University

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We present a protocol for implementing interference-reflection microscopy and total-internal-reflection-fluorescence microscopy for the simultaneous imaging of dynamic microtubules and fluorescently labeled microtubule-associated proteins.

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Simultaneous Interference Reflection Total Internal Reflection Fluorescence Microscopy Dynamic Microtubules Associated Proteins Label-free Microtubules Fluorescently Labeled Proteins High Frame Rates Easy Implementation Minimal Demands On Optical Components Fluoro-4 Labeling Of Microtubules PDMS Polymer Curing Agent Base Elastomer Mass Ratio Vacuum Chamber Master Mold Bubble Avoidance Technique Preheated Oven Structured Regions Of The Polymer PDMS Puncher Plasma Cleaning Oxygen Plasma Air Plasma Cover Glass Hot Plate Low-density Polyethylene Tubing
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