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Journal / Bioengineering / 开发用于心脏功能成像和光遗传学控制的 黑腹果蝇…
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Bioengineering

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JoVE Journal Bioengineering
开发用于心脏功能成像和光遗传学控制的 黑腹果蝇 模型
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开发用于心脏功能成像和光遗传学控制的 黑腹果蝇 模型

Article DOI: 10.3791/63939-v • 08:43 min • August 25th, 2022
August 25th, 2022 •
1, 1, 1, 1, 1,2, 1
1Department of Biomedical Engineering, Washington University in St. Louis, 2Department of Computer Science and Engineering, Washington University in St. Louis

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本方案描述了在心脏中表达eNpHR2.0或ReaChR视蛋白的黑色果蝇的产生，用于OCT成像和光遗传学心脏起搏。报告了果蝇OCT成像和心跳调节的详细说明，包括模拟不同发育阶段活体动物的可恢复性心骤停，心动过缓和心动过速。

Tags

生物工程， 第186期， 黑色腹果蝇， OCT， 光遗传学， 心脏骤停， 心动过缓， 心动过速， 心脏起搏， 视蛋白， eNpHR2.0， ReaChR
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