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Journal / Biochemistry / 修飾酵素結合免疫吸着アッセイを用いたヒト血漿中のカルシトニン遺伝子関連ペプチド(CGRP)の検出と定…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
修飾酵素結合免疫吸着アッセイを用いたヒト血漿中のカルシトニン遺伝子関連ペプチド(CGRP)の検出と定量(英語)
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Journal (Biochemistry)
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
Journal (Biochemistry)
Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
 
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修飾酵素結合免疫吸着アッセイを用いたヒト血漿中のカルシトニン遺伝子関連ペプチド(CGRP)の検出と定量(英語)

Article DOI: 10.3791/64775-v • 07:14 min • June 16th, 2023
June 16th, 2023 •
1,2, 1,3, 1, 4, 5, 1, 6, 1,3, 1
1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine

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ヒト血漿中のカルシトニン遺伝子関連ペプチド(CGRP)濃度に関する公表されたデータは一貫していません。.これらの不一致は、この神経ペプチドを定量するための標準化された検証済みの方法論の欠如が原因である可能性があります。ここでは、ヒト血漿中のCGRPを精製および定量するための検証済みの酵素結合免疫吸着アッセイ(ELISA)プロトコルについて説明します。

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今月のJoVE、第196号、
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