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Biochemistry

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JoVE Journal Biochemistry
Detektion och kvantifiering av kalcitoningenrelaterad peptid (CGRP) i human plasma med hjälp av en modifierad enzymkopplad immunadsorberande analys
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Detektion och kvantifiering av kalcitoningenrelaterad peptid (CGRP) i human plasma med hjälp av en modifierad enzymkopplad immunadsorberande analys

Article DOI: 10.3791/64775-v • 07:14 min • June 16th, 2023
June 16th, 2023 •
1,2, 1,3, 1, 4, 5, 1, 6, 1,3, 1
1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine

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Publicerade data avseende koncentrationer av kalcitonin-genrelaterade peptider (CGRP) i human plasma är inkonsekventa. Dessa inkonsekvenser kan bero på bristen på en standardiserad, validerad metod för att kvantifiera denna neuropeptid. Här beskriver vi ett validerat enzymkopplat immunosorbentanalysprotokoll (ELISA) för att rena och kvantifiera CGRP i human plasma.

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Denna månad i JoVE nummer 196
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