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Journal / Biochemistry / Nachweis und Quantifizierung von Calcitonin…
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Biochemistry

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JoVE Journal Biochemistry
Nachweis und Quantifizierung von Calcitonin Gene-Related Peptide (CGRP) in humanem Plasma mit Hilfe eines modifizierten Enzyme-linked Immunosorbent Assays
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Nachweis und Quantifizierung von Calcitonin Gene-Related Peptide (CGRP) in humanem Plasma mit Hilfe eines modifizierten Enzyme-linked Immunosorbent Assays

Article DOI: 10.3791/64775-v • 07:14 min • June 16th, 2023
June 16th, 2023 •
1,2, 1,3, 1, 4, 5, 1, 6, 1,3, 1
1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine

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Publizierte Daten zu Calcitonin-Gen-Related Peptide (CGRP)-Konzentrationen im menschlichen Plasma sind widersprüchlich. Diese Inkonsistenzen könnten auf das Fehlen einer standardisierten, validierten Methodik zur Quantifizierung dieses Neuropeptids zurückzuführen sein. In dieser Arbeit beschreiben wir ein validiertes ELISA-Protokoll (Enzyme-linked Immunosorbent Assay) zur Aufreinigung und Quantifizierung von CGRP in humanem Plasma.

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