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Journal / Biochemistry / "Cell Surface Capture" workflow voor labelvrije…
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Biochemistry

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JoVE Journal Biochemistry
"Cell Surface Capture" workflow voor labelvrije kwantificering van het celoppervlakproteoom
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
Journal (Biochemistry)
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE
Journal (Biochemistry)
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE
 
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"Cell Surface Capture" workflow voor labelvrije kwantificering van het celoppervlakproteoom

Article DOI: 10.3791/64952-v • 06:31 min • March 24th, 2023
March 24th, 2023 •
1, 1,2, 1,3,4
1Department of Laboratory Medicine, University of California, San Francisco, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 4Chan Zuckerberg Biohub

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Hier beschrijven we een proteomics-workflow voor de karakterisering van het celoppervlakproteoom van verschillende celtypen. Deze workflow omvat eiwitverrijking van het celoppervlak, daaropvolgende monstervoorbereiding, analyse met behulp van een LC-MS / MS-platform en gegevensverwerking met gespecialiseerde software.

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Biochemie Nummer 193
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