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Journal / Biochemistry / "Cell Surface Capture" arbetsflöde för etikettfri …
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Biochemistry

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JoVE Journal Biochemistry
"Cell Surface Capture" arbetsflöde för etikettfri kvantifiering av cellyteproteomet
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"Cell Surface Capture" arbetsflöde för etikettfri kvantifiering av cellyteproteomet

Article DOI: 10.3791/64952-v • 06:31 min • March 24th, 2023
March 24th, 2023 •
1, 1,2, 1,3,4
1Department of Laboratory Medicine, University of California, San Francisco, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 4Chan Zuckerberg Biohub

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Här beskriver vi ett proteomikarbetsflöde för karakterisering av cellyteproteomet hos olika celltyper. Detta arbetsflöde inkluderar proteinanrikning på cellytan, efterföljande provberedning, analys med hjälp av en LC-MS / MS-plattform och databehandling med specialiserad programvara.

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Biokemi nummer 193
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