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Journal / Biochemistry / "Cell Surface Capture" workflow til etiketfri…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
"Cell Surface Capture" workflow til etiketfri kvantificering af celleoverfladeproteomet
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Journal (Biochemistry)
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Journal (Biochemistry)
Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
 
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"Cell Surface Capture" workflow til etiketfri kvantificering af celleoverfladeproteomet

Article DOI: 10.3791/64952-v • 06:31 min • March 24th, 2023
March 24th, 2023 •
1, 1,2, 1,3,4
1Department of Laboratory Medicine, University of California, San Francisco, 2Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, 3Deptartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, 4Chan Zuckerberg Biohub

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Her beskriver vi en proteomics arbejdsgang til karakterisering af celleoverfladeproteomet af forskellige celletyper. Denne arbejdsgang omfatter celleoverfladeproteinberigelse, efterfølgende prøveforberedelse, analyse ved hjælp af en LC-MS / MS-platform og databehandling med specialiseret software.

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Biokemi nr. 193
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