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Journal / Biochemistry / Enkeltmolekylebilleddannelse af lateral mobilitet …
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Biochemistry

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JoVE Journal Biochemistry
Enkeltmolekylebilleddannelse af lateral mobilitet og ionkanalaktivitet i lipiddobbeltlag ved hjælp af total intern refleksionsfluorescens (TIRF) mikroskopi
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Journal (Biochemistry)
Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Journal (Biochemistry)
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Enkeltmolekylebilleddannelse af lateral mobilitet og ionkanalaktivitet i lipiddobbeltlag ved hjælp af total intern refleksionsfluorescens (TIRF) mikroskopi

Article DOI: 10.3791/64970-v • 08:55 min • February 17th, 2023
February 17th, 2023 •
1, 1
1Biophysics Department, Institute of Biomaterials and Biomolecular Systems, University of Stuttgart

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Denne protokol beskriver, hvordan man bruger TIRF-mikroskopi til at spore individuelle ionkanaler og bestemme deres aktivitet i understøttede lipidmembraner og derved definere samspillet mellem lateral membranbevægelse og kanalfunktion. Den beskriver, hvordan man forbereder membranerne, registrerer dataene og analyserer resultaterne.

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Biokemi nr. 192
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