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הארכת תוחלת החיים של נויטרופילים עם CLON-G ובדיקת מוות ספונטני במבחנה

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster

Journal (Biology)

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster

Measuring Sarcoplasmic Calcium Concentration in Isolated Mouse Muscle Fibers

Journal (Biology)

Measuring Sarcoplasmic Calcium Concentration in Isolated Mouse Muscle Fibers

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הארכת תוחלת החיים של נויטרופילים עם CLON-G ובדיקת מוות ספונטני במבחנה

Article DOI: 10.3791/65132-v • 05:52 min • May 12th, 2023

May 12th, 2023 •

Yuping Fan*^1,2, Yan Teng*³, Fu tong Liu*⁴, Fengxia Ma^1,2, Alan Y. Hsu⁵, Sizhou Feng^1,2, Hongbo R. Luo⁵

¹State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, ²Tianjin Institutes of Health Science, ³Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, ⁴Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, ⁵Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center

* These authors contributed equally

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פרוטוקול זה מפרט את הכנת CLON-G להארכת תוחלת החיים של נויטרופילים ליותר מ-5 ימים ומספק הליך אמין להערכת מוות נויטרופילים באמצעות ציטומטריית זרימה ומיקרוסקופ פלואורסצנטי קונפוקלי.

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