Name: Generazione 3D di tessuto miocardico umano mediante scrittura di elettrofilatura per fusione di scaffold di policaprolattone e cellule cardiache derivate da hiPSC (Video) | JoVE Journal
Uploaded: 2025-03-28T14:45:00.000+00:00
Duration: 6 min 17 s
Description: Impara a creare tessuti cardiaci 3D con melt electrospinning writing e cellule hiPSC per modellare malattie e testare farmaci. Questo metodo migliora la rilevanza rispetto alle colture 2D.

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Questa ricerca è stata finanziata dal programma di ricerca e innovazione H2020 nell'ambito delle convenzioni di sovvenzione n. 874827 (BRAV);<img alt="Equation 1" src="/files/ftp_upload/67847/67847eq01.jpg" style="margin: auto;"> Ministerio de Ciencia y Universidades (Spagna) attraverso i progetti PLEC2021-008127 (CARDIOPRINT), PID2022-142562OB-I00 (VOLVAD) e PID2022-142807OA-I00 (INVESTTRA) finanziati da MICIU/AEI/10.13039/501100011033 e dall'Unione Europea NextGenerationEU/PRTR; Gobierno de Navarra Proyectos Estratégicos IMPRIMED (0011-1411-2021-000096) e BIOHEART (0011-1411-2022-000071); Gobierno de Navarra Proyectos Colaborativos BIOGEN (PC020-021-022) e Gobierno de Navarra Salud GN32/2023. Figure 1A, Figure 2A e Figure 4A vengono create con BioRender.com.

<ol><li>Cho, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Challenges+and+opportunities+for+the+next+generation+of+cardiovascular+tissue+engineering%5BTitle%5D)+AND+%22Nat+Methods%22%5BJournal%5D)">Challenges and opportunities for the next generation of cardiovascular tissue engineering.</a> Nat Methods. 19 (9), 1064-1071 (2022).</li>
<li>Garbern, J. C., Lee, R. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Heart+regeneration%3A+20+years+of+progress+and+renewed+optimism%5BTitle%5D)+AND+%22Dev+Cell%22%5BJournal%5D)">Heart regeneration: 20 years of progress and renewed optimism.</a> Dev Cell. 57 (4), 424-439 (2022).</li>
<li>Lian, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Robust+cardiomyocyte+differentiation+from+human+pluripotent+stem+cells+via+temporal+modulation+of+canonical+Wnt+signaling%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+USA%22%5BJournal%5D)">Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling.</a> Proc Natl Acad Sci USA. 109 (27), E1848-E1857 (2012).</li>
<li>Burridge, P. W., Holmström, A., Wu, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chemically+defined+culture+and+cardiomyocyte+differentiation+of+human+pluripotent+stem+cells%5BTitle%5D)+AND+%22Curr+Protoc+Hum+Genet%22%5BJournal%5D)">Chemically defined culture and cardiomyocyte differentiation of human pluripotent stem cells.</a> Curr Protoc Hum Genet. 87 (21), 21.3.1-21.3.15 (2015).</li>
<li>Feric, N. T., Radisic, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Maturing+human+pluripotent+stem+cell-derived+cardiomyocytes+in+human+engineered+cardiac+tissues%5BTitle%5D)+AND+%22Adv+Drug+Deliv+Rev%22%5BJournal%5D)">Maturing human pluripotent stem cell-derived cardiomyocytes in human engineered cardiac tissues.</a> Adv Drug Deliv Rev. 96, 110-134 (2016).</li>
<li>Huang, S., Yang, Y., Yang, Q., Zhao, Q., Ye, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Engineered+circulatory+scaffolds+for+building+cardiac+tissue%5BTitle%5D)+AND+%22J+Thorac+Dis%22%5BJournal%5D)">Engineered circulatory scaffolds for building cardiac tissue.</a> J Thorac Dis. 10 (Suppl 20), S2312-S2328 (2018).</li>
<li>Pinto, A. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Revisiting+cardiac+cellular+composition%5BTitle%5D)+AND+%22Circ+Res%22%5BJournal%5D)">Revisiting cardiac cellular composition.</a> Circ Res. 118 (3), 400-409 (2016).</li>
<li>Brown, T. D., Dalton, P. D., Hutmacher, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Direct+writing+by+way+of+melt+electrospinning%5BTitle%5D)+AND+%22Adv+Mater%22%5BJournal%5D)">Direct writing by way of melt electrospinning.</a> Adv Mater. 23 (47), 5651-5657 (2011).</li>
<li>Vernon, M. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Engineering+heart+valve+interfaces+using+melt+electrowriting%3A+Biomimetic+design+strategies+from+multi-modal+imaging%5BTitle%5D)+AND+%22Adv+Healthc+Mater%22%5BJournal%5D)">Engineering heart valve interfaces using melt electrowriting: Biomimetic design strategies from multi-modal imaging.</a> Adv Healthc Mater. 11 (24), 2201028(2022).</li>
<li>Montero-Calle, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Fabrication+of+human+myocardium+using+multidimensional+modeling+of+engineered+tissues%5BTitle%5D)+AND+%22Biofabrication%22%5BJournal%5D)">Fabrication of human myocardium using multidimensional modeling of engineered tissues.</a> Biofabrication. 14 (4), 045017(2022).</li>
<li>Castilho, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Melt+electrospinning+writing+of+poly-hydroxymethylglycolide-co-%CE%B5-caprolactone-based+scaffolds+for+cardiac+tissue+engineering%5BTitle%5D)+AND+%22Adv+Healthc+Mater%22%5BJournal%5D)">Melt electrospinning writing of poly-hydroxymethylglycolide-co-ε-caprolactone-based scaffolds for cardiac tissue engineering.</a> Adv Healthc Mater. 6 (18), 1700311(2017).</li>
<li>Castilho, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Melt+electrowriting+allows+tailored+microstructural+and+mechanical+design+of+scaffolds+to+advance+functional+human+myocardial+tissue+formation%5BTitle%5D)+AND+%22Adv+Funct+Mater%22%5BJournal%5D)">Melt electrowriting allows tailored microstructural and mechanical design of scaffolds to advance functional human myocardial tissue formation.</a> Adv Funct Mater. 28 (40), 1803151(2018).</li>
<li>Ronaldson-Bouchard, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advanced+maturation+of+human+cardiac+tissue+grown+from+pluripotent+stem+cells%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Advanced maturation of human cardiac tissue grown from pluripotent stem cells.</a> Nature. 556, 239-243 (2018).</li>
<li>Palaskas, N. L., Ali, H. J., Koutroumpakis, E., Ganatra, S., Deswal, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cardiovascular+toxicity+of+immune+therapies+for+cancer%5BTitle%5D)+AND+%22BMJ%22%5BJournal%5D)">Cardiovascular toxicity of immune therapies for cancer.</a> BMJ. 385, e075859(2024).</li>
<li>Lian, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Directed+cardiomyocyte+differentiation+from+human+pluripotent+stem+cells+by+modulating+Wnt%2F%CE%B2-catenin+signaling+under+fully+defined+conditions%5BTitle%5D)+AND+%22Nat+Protoc%22%5BJournal%5D)">Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions.</a> Nat Protoc. 8 (1), 162-175 (2012).</li>
<li>Zhang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Generation+of+quiescent+cardiac+fibroblasts+from+human+induced+pluripotent+stem+cells+for+in+vitro+modeling+of+cardiac+fibrosis%5BTitle%5D)+AND+%22Circ+Res%22%5BJournal%5D)">Generation of quiescent cardiac fibroblasts from human induced pluripotent stem cells for in vitro modeling of cardiac fibrosis.</a> Circ Res. 125 (5), 552-566 (2019).</li>
<li>Breckwoldt, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differentiation+of+cardiomyocytes+and+generation+of+human-engineered+heart+tissue%5BTitle%5D)+AND+%22Nat+Protoc%22%5BJournal%5D)">Differentiation of cardiomyocytes and generation of human-engineered heart tissue.</a> Nat Protoc. 12 (6), 1177-1197 (2017).</li>
<li>Ronaldson-Bouchard, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Advanced+maturation+of+human+cardiac+tissue+grown+from+pluripotent+stem+cells%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Advanced maturation of human cardiac tissue grown from pluripotent stem cells.</a> Nature. 556, 239(2018).</li>
<li>Rivera-Arbeláez, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Automated+assessment+of+human-engineered+heart+tissues+using+deep+learning+and+template+matching+for+segmentation+and+tracking%5BTitle%5D)+AND+%22Bioeng+Transl+Med%22%5BJournal%5D)">Automated assessment of human-engineered heart tissues using deep learning and template matching for segmentation and tracking.</a> Bioeng Transl Med. 8 (3), e10513(2023).</li>
<li>Sala, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((MUSCLEMOTION%3A+A+versatile+open+software+tool+to+quantify+cardiomyocyte+and+cardiac+muscle+contraction+in+vitro+and+in+vivo%5BTitle%5D)+AND+%22Circ+Res%22%5BJournal%5D)">MUSCLEMOTION: A versatile open software tool to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo.</a> Circ Res. 122 (3), e5-e16 (2018).</li>
<li>Reyat, J. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Modeling+the+pathology+and+treatment+of+cardiac+fibrosis+in+vascularised+atrial+and+ventricular+cardiac+microtissues%5BTitle%5D)+AND+%22Front+Cardiovasc+Med%22%5BJournal%5D)">Modeling the pathology and treatment of cardiac fibrosis in vascularised atrial and ventricular cardiac microtissues.</a> Front Cardiovasc Med. 10, 1156759(2023).</li>
<li>Park, J. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Materials+and+extracellular+matrix+rigidity+highlighted+in+tissue+damages+and+diseases%3A+Implication+for+biomaterials+design+and+therapeutic+targets%5BTitle%5D)+AND+%22Bioact+Mater%22%5BJournal%5D)">Materials and extracellular matrix rigidity highlighted in tissue damages and diseases: Implication for biomaterials design and therapeutic targets.</a> Bioact Mater. 20, 381-403 (2023).</li>
<li>Wunner, F. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Melt+electrospinning+writing+of+three-dimensional+poly%28%CE%B5-caprolactone%29+scaffolds+with+controllable+morphologies+for+tissue+engineering+applications%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Melt electrospinning writing of three-dimensional poly(ε-caprolactone) scaffolds with controllable morphologies for tissue engineering applications.</a> J Vis Exp. 130, e55617(2017).</li>
<li>Robinson, T. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+next+frontier+in+melt+electrospinning%3A+Taming+the+jet%5BTitle%5D)+AND+%22Adv+Funct+Mater%22%5BJournal%5D)">The next frontier in melt electrospinning: Taming the jet.</a> Adv Funct Mater. 29 (44), 1904664(2019).</li>
<li>Kade, J. C., Dalton, P. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Polymers+for+MELT+electrowriting%5BTitle%5D)+AND+%22Adv+Healthc+Mater%22%5BJournal%5D)">Polymers for MELT electrowriting.</a> Adv Healthc Mater. 10 (1), 2001232(2021).</li>
<li>Inchingolo, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Polycaprolactone+in+bone+tissue+engineering%3A+A+comprehensive+review+of+innovations+in+scaffold+fabrication+and+surface+modifications%5BTitle%5D)+AND+%22J+Funct+Biomater%22%5BJournal%5D)">Polycaprolactone in bone tissue engineering: A comprehensive review of innovations in scaffold fabrication and surface modifications.</a> J Funct Biomater. 15 (9), 243(2024).</li>
<li>Mondadori, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessing+the+response+of+human+primary+macrophages+to+defined+fibrous+architectures+fabricated+by+melt+electrowriting%5BTitle%5D)+AND+%22Bioact+Mater%22%5BJournal%5D)">Assessing the response of human primary macrophages to defined fibrous architectures fabricated by melt electrowriting.</a> Bioact Mater. 21, 209-222 (2022).</li>
<li>Xu, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Melt-electrowriting-enabled+anisotropic+scaffolds+loaded+with+valve+interstitial+cells+for+heart+valve+tissue+Engineering%5BTitle%5D)+AND+%22J+Nanobiotechnology%22%5BJournal%5D)">Melt-electrowriting-enabled anisotropic scaffolds loaded with valve interstitial cells for heart valve tissue Engineering.</a> J Nanobiotechnology. 22 (1), 378(2024).</li>
<li>Ristovski, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Improved+fabrication+of+melt+electrospun+tissue+engineering+scaffolds+using+direct+writing+and+advanced+electric+field+control%5BTitle%5D)+AND+%22Biointerphases%22%5BJournal%5D)">Improved fabrication of melt electrospun tissue engineering scaffolds using direct writing and advanced electric field control.</a> Biointerphases. 10 (1), 011006(2015).</li>
<li>Puluca, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bioprinting+approaches+to+engineering+vascularized+3D+cardiac+tissues%5BTitle%5D)+AND+%22Curr+Cardiol+Rep%22%5BJournal%5D)">Bioprinting approaches to engineering vascularized 3D cardiac tissues.</a> Curr Cardiol Rep. 21 (9), 1-11 (2019).</li>
<li>Lu, T. Y., Xiang, Y., Tang, M., Chen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((3D+printing+approaches+to+engineer+cardiac+tissue%5BTitle%5D)+AND+%22Curr+Cardiol+Rep%22%5BJournal%5D)">3D printing approaches to engineer cardiac tissue.</a> Curr Cardiol Rep. 25 (6), 505-514 (2023).</li>
<li>Voges, H. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vascular+cells+improve+functionality+of+human+cardiac+organoids%5BTitle%5D)+AND+%22Cell+Rep%22%5BJournal%5D)">Vascular cells improve functionality of human cardiac organoids.</a> Cell Rep. 42 (5), 112322(2023).</li>
<li>Zhu, L., Liu, K., Feng, Q., Liao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cardiac+organoids%3A+A+3D+technology+for+modeling+heart+development+and+disease%5BTitle%5D)+AND+%22Stem+Cell+Rev+Rep%22%5BJournal%5D)">Cardiac organoids: A 3D technology for modeling heart development and disease.</a> Stem Cell Rev Rep. 18 (8), 2593-2605 (2022).</li>
<li>Karbassi, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cardiomyocyte+maturation%3A+advances+in+knowledge+and+implications+for+regenerative+medicine%5BTitle%5D)+AND+%22Nat+Rev+Cardiol%22%5BJournal%5D)">Cardiomyocyte maturation: advances in knowledge and implications for regenerative medicine.</a> Nat Rev Cardiol. 17 (6), 341-359 (2020).</li>
<li>Carlos-Oliveira, M., Lozano-Juan, F., Occhetta, P., Visone, R., Rasponi, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Current+strategies+of+mechanical+stimulation+for+maturation+of+cardiac+microtissues%5BTitle%5D)+AND+%22Biophys+Rev%22%5BJournal%5D)">Current strategies of mechanical stimulation for maturation of cardiac microtissues.</a> Biophys Rev. 13 (5), 717(2021).</li>
<li>Maihemuti, W., Murata, K., Abulaiti, M., Minatoya, K., Masumoto, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Simultaneous+electro-dynamic+stimulation+accelerates+maturation+of+engineered+cardiac+tissues+generated+by+human+iPS+cells%5BTitle%5D)+AND+%22Biochem+Biophys+Res+Commun%22%5BJournal%5D)">Simultaneous electro-dynamic stimulation accelerates maturation of engineered cardiac tissues generated by human iPS cells.</a> Biochem Biophys Res Commun. 733, 150605(2024).</li>
<li>Wang, L., Serpooshan, V., Zhang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Engineering+human+cardiac+muscle+patch+constructs+for+prevention+of+post-infarction+LV+remodeling%5BTitle%5D)+AND+%22Front+Cardiovasc+Med%22%5BJournal%5D)">Engineering human cardiac muscle patch constructs for prevention of post-infarction LV remodeling.</a> Front Cardiovasc Med. 8, 621781(2021).</li>
<li>Matveeva, V. G., Khanova, M. U., Antonova, L. V., Barbarash, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Fibrin+%E2%80%93+A+promising+material+for+vascular+tissue+engineering%5BTitle%5D)+AND+%22Russ+J+Transplantol+Artif+Organs%22%5BJournal%5D)">Fibrin – A promising material for vascular tissue engineering.</a> Russ J Transplantol Artif Organs. 22 (1), 196-208 (2020).</li>
<li>Sproul, E. P., Hannan, R. T., Brown, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Controlling+fibrin+network+morphology%2C+polymerization%2C+and+degradation+dynamics+in+fibrin+gels+for+promoting+tissue+repair%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Controlling fibrin network morphology, polymerization, and degradation dynamics in fibrin gels for promoting tissue repair.</a> Methods Mol Biol. 1758, 85-99 (2018).</li>
</ol>

<table><tbody><tr><td>&micro;-Slide Vetrino coprioggetto camera a 8 pozzetti (IbiTreat)</td><td>IBIDI</td><td>80826</td><td></td></tr><tr><td>Soluzione di gelatina allo 0,1% (Embryomax)</td><td>Merck Millipore</td><td>ES-006-B</td><td></td></tr><tr><td>10% formalina</td><td>Sigma Aldrich</td><td>HT501128</td><td></td></tr><tr><td>piastre a 12 pozzetti</td><td>Costar/Corning</td><td>3513</td><td></td></tr><tr><td>piastre a 6 pozzetti</td><td>Costar/Corning</td><td>3506</td><td></td></tr><tr><td>Advanced DMEM 1x (ADMEM)</td><td>Gibco</td><td>12491015</td><td></td></tr><tr><td>Aprotinina da polmone bovino</td><td>Sigma Aldrich</td><td>A1153</td><td></td></tr><tr><td>B-27 SUPLEMENT, PLUS INSULIN (50x)&nbsp;</td><td>Life Technologies</td><td>A317504044</td><td></td></tr><tr><td>INTEGRATORE DI B27, SENZA INSULINA (50x)</td><td>Life Technologies</td><td>A1895601</td><td></td></tr><tr><td>Punzone per biopsia (diametro 6 mm)</td><td>Medical</td><td>BP-60F</td><td></td></tr><tr><td>Albumina sierica bovina (BSA)</td><td>Sigma Aldrich</td><td>A9647</td><td></td></tr><tr><td>CHIR-99021&nbsp;</td><td>AXON MEDCHEM</td><td>AXON1386</td><td></td></tr><tr><td>Microscopio confocale a scansione laser</td><td>Zeiss</td><td>LSM 800</td><td></td></tr><tr><td>Pallone di coltura 175 cm</td><td>Greiner Bio-One</td><td>660175</td><td></td></tr><tr><td>Pallone di coltura 75 cm</td><td>Falcon</td><td>353136</td><td></td></tr><tr><td>citometro</td><td>Beckman Coulter</td><td>CytoFlex&nbsp;</td><td></td></tr><tr><td>DAPI&nbsp;</td><td>Sigma Aldrich</td><td>D9542</td><td></td></tr><tr><td>Asino anti-topo IgG (H + L) Anticorpo secondario altamente adsorbito, Alexa Fluor 488</td><td>Invitrogen</td><td>A-21202</td><td></td></tr><tr><td>Anticorpo secondario altamente adsorbito anti-coniglio IgG (H+L) dell'asino, Alexa Fluor 594</td><td>Invitrogen</td><td>A-21207&nbsp;</td><td></td></tr><tr><td>DPBS (senza Mg2+, Ca2+)</td><td>Gibco</td><td>A314190094</td><td></td></tr><tr><td>EDTA 0,5M pH 8,0</td><td>Life Technologies</td><td>AM9260G</td><td></td></tr><tr><td>KIT ESSENTIAL 8 MEDIUM Life Technologies</td><td></td><td>A1517001</td><td></td></tr><tr><td>FGF2 (FGF-basico umano ricombinante 154 a.a.)</td><td>Peprotech</td><td>100-18B</td><td></td></tr><tr><td>Fibrinogeno da plasma bovino</td><td>Sigma Aldrich</td><td>F8630</td><td></td></tr><tr><td>Terreno di crescita dei fibroblasti 3 KIT (FGM3)</td><td>PromoCell</td><td>C-23130</td><td></td></tr><tr><td>Knockout Serum Replacement (KSR)</td><td>Life Technologies</td><td>10828028</td><td></td></tr><tr><td>Lattato (L-lattato di sodio)</td><td>Sigma Aldrich</td><td>71718</td><td></td></tr><tr><td>Matrice della membrana basale con fattore di crescita ridotto (MGFR) Matrigel, senza LDEV</td><td>Corning</td><td>354230</td><td></td></tr><tr><td></td><td>MEW Ago 23-G</td><td>Stampante MEW Nordson</td> 7018302 <td></td></tr><tr><td>QUT</td><td>, Queensland University of Technology</td><td></td><td></td></tr><tr><td>Siringa MEW</td><td>Nordson</td><td>7012072</td><td></td></tr><tr><td>Anticorpo monoclonale anti-troponina T cardiaca di topo</td><td>Invitrogen</td><td>MA5-12960&nbsp;</td><td></td></tr><tr><td>Anticorpo monoclonale anti-DDR2 di topo</td><td>Sigma Aldrich</td><td>SAB5300116</td><td></td></tr><tr><td>Anticorpo monoclonale anti-alfa-alfa;-actinina (sarcomerico) di topo</td><td>Sigma Aldrich</td><td>A7811&nbsp;</td><td></td></tr><tr><td>PENICILLINA - STREPTOMICINA</td><td>Life Technologies</td><td>15140122</td><td></td></tr><tr><td>Poli &epsilon;-caprolattone (PCL), grado medico</td><td>Corbion</td><td>PURASORB&reg; PC 12</td><td></td></tr><tr><td>Anticorpo monoclonale ricombinante anti-vimentina di coniglio [EPR3776] - marcatore citoscheletro</td><td>Abcam</td><td>Ab29547&nbsp;</td><td></td></tr><tr><td>Acido retinoico&nbsp;</td><td>Sigma Aldrich</td><td>R2625</td><td></td></tr><tr><td>ROCK inibitore Y-27632 (10mg)</td><td>Fisher Scientific</td><td>HB2297</td><td></td></tr><tr><td>RPMI 1640 (L-glutammina)</td><td>Gibco</td><td>21875034</td><td></td></tr><tr><td>RPMI 1640 (senza rosso fenolo)</td><td>Gibco</td><td>32404014</td><td></td></tr><tr><td>RPMI senza glucosio</td><td>Gibco</td><td>11879020</td><td></td></tr><tr><td>SB431542</td><td>SELLECK CHEMICALS</td><td>S1067</td><td></td></tr><tr><td>Spettrofotometro&nbsp;</td><td>BMG Labtech&nbsp;</td><td>SPECTROstar Nano</td><td></td></tr><tr><td>Trombina (alfa trombina umana, fattore IIa)Laboratorio</td> <td>di ricerca enzimatica</td><td>HT 1002a</td><td></td></tr><tr><td>Triton X-100</td><td>Sigma Aldrich</td><td>T8787</td><td></td></tr><tr><td>TrypLE Express</td><td>Life Technologies</td><td>12604021</td><td></td></tr><tr><td>Tween 20</td><td>Sigma Aldrich</td><td>P2287</td><td></td></tr><tr><td>Wnt-C59</td><td>AXON MEDCHEM</td><td>AXON2287</td><td></td></tr></tbody></table>

generazione 3d di tessuto miocardico umano mediante scrittura di elettrofilatura per fusione di scaffold di policaprolattone e cellule cardiache derivate da hipsc

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Caratterizzazione di cellule cardiache derivate da hiPSC 2D Al fine di generare tessuti cardiaci multifenotipici, le hiPSC-CM e le hiPSC-CF sono differenziate in modo indipendente e caratterizzate in vitro. Con un'appropriata ottimizzazione e una manutenzione rigorosa, il seguente protocollo si tradurrà in una resa di hiPSC-CM di oltre l'80% intorno al giorno 9 di differenziazione, dando origine a cellule che battono spontaneamente (Figura 1A). Inoltre, l'arricch...

Generazione 3D di tessuto miocardico umano mediante scrittura di elettrofilatura per fusione di scaffold di policaprolattone e cellule cardiache derivate da hiPSC

The development of functional human cardiac tissues holds significant promise for advancing applications in drug screening, disease modeling, and ...

3d-human-myocardial-tissue-generation-using-melt-electrospinning

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JoVE Journal

Bioengineering

Bioingegneria

La generazione di allineato tessuto miocardico è un requisito fondamentale per adattare i recenti progressi nel campo della biologia delle cellule staminali a fini clinicamente utili. Qui si descrive un metodo di stampa per microcontatto il controllo preciso di forma e funzione cellulare. Utilizzo di popolazioni altamente purificate di cellule staminali embrionali derivate progenitori cardiaci, abbiamo quindi generare anisotropo tessuto funzionale del miocardio.

Generazione di Allineati tessuto funzionale del miocardio attraverso la stampa microcontatto

Questo manoscritto descrive la creazione di tessuti cardiaci ingegnerizzati definiti utilizzando l'espressione di marcatori di superficie e la selezione cellulare. I tessuti definiti possono quindi essere utilizzati in un bioreattore multi-tessuto per studiare i meccanismi della terapia cellulare cardiaca al fine di fornire un sistema modello funzionale, ma controllato, del cuore umano.

Costruzione di Engineered Definito umani cardiache tessuti per studiare i meccanismi della terapia cellulare cardiaca

Questo protocollo descrive la bioprinting 3D del tessuto cardiaco senza l'uso di biomateriali. Le zone cardiache 3D bioprinted espongono l'integrazione meccanica dei sferoidi dei componenti e sono altamente promettenti nella rigenerazione del tessuto cardiaco e come modelli 3D di malattie cardiache.

Creazione di tessuto cardiaco che mostra l&#39;integrazione meccanica degli spheroidi usando la biopittura 3D

Questo protocollo descrive un metodo basato su stampo netto per creare tessuti cardiaci senza impalcatura tridimensionale con soddisfacente integrità strutturale e comportamento sincrono pestaggio.

Un metodo basato su stampo netto della creazione senza impalcatura tridimensionale del tessuto cardiaco

L'attuale protocollo genera tessuti cardiaci ingegnerizzati a forma di maglia contenenti cellule cardiovascolari derivate da cellule staminali pluripotenti indotte dall'uomo per consentire lo studio della terapia dell'impianto cellulare per le malattie cardiache.

Preparazione di tessuti cardiaci ingegnerizzati a forma di mesh derivati da cellule iPS umane per la riparazione miocardia in Vivo

Qui, descriviamo una metodologia facile da usare per generare array di microtessuti cardiaci auto-assemblati 3D composti da cardiomiociti derivati da cellule staminali pluripotenti preseferenziate indotte dall'uomo, fibroblasti cardiaci e cellule endoteliali. Questa tecnica user-friendly e a bassa richiesta cellulare per generare microtessuti cardiaci può essere implementata per la modellazione della malattia e le prime fasi dello sviluppo del farmaco.

Fabbricazione di array di microtissue cardiaci 3D utilizzando cardiomiociti derivati da iPSC umani, fibroblasti cardiaci e cellule endoteliali

L'obiettivo di questo protocollo è spiegare e dimostrare lo sviluppo di un modello microfluidico tridimensionale (3D) di tessuto cardiaco umano altamente allineato, composto da cardiomiociti derivati da cellule staminali co-coltivati con fibroblasti cardiaci (FF) all'interno di un idrogel biomimetico a base di collagene, per applicazioni nell'ingegneria dei tessuti cardiaci, nello screening dei farmaci e nella modellazione delle malattie.

Sviluppo di tessuto cardiaco umano organizzato in 3D all'interno di una piattaforma microfluidico

Il metodo presentato offre un modo innovativo per l'ingegneria di strutture in fibre biomimetiche in scaffold tridimensionali (3D) (ad esempio, volantini di valvole cardiache). Le geometrie conduttive stampate in 3D sono state utilizzate per determinare forma e dimensioni. L'orientamento e le caratteristiche delle fibre sono stati regolabili individualmente per ogni strato. Più campioni potrebbero essere prodotti in un'unica configurazione.

Combinazione di stampa 3D ed elettrofilatura per la produzione di volantini biomimetici per valvole cardiache

Qui viene presentato un insieme di protocolli per la generazione e la crioconservazione di sferoidi cardiaci (CS) da cardiomiociti derivati da cellule staminali pluripotenti indotti dall'uomo coltivati in un formato multidimensionale ad alto rendimento. Questo modello tridimensionale funziona come una solida piattaforma per la modellazione delle malattie, gli screening ad alto rendimento e mantiene la sua funzionalità dopo la crioconservazione.

Generazione, screening ad alto rendimento e biobanca di sferoidi cardiaci derivati da cellule staminali pluripotenti indotte dall'uomo

L'uso della luce per controllare le cellule e i tessuti cardiaci consente la stimolazione senza contatto, preservando così lo stato naturale e la funzione delle cellule, rendendolo un approccio prezioso sia per la ricerca di base che per le applicazioni terapeutiche.

Un sistema microfisiologico cardiaco per lo studio della propagazione del Ca2+ tramite stimolazione ottica non genetica

Impara a creare tessuti cardiaci 3D con melt electrospinning writing e cellule hiPSC per modellare malattie e testare farmaci. Questo metodo migliora la rilevanza rispetto alle colture 2D.

Generazione 3D di tessuto miocardico umano mediante scrittura di elettrofilatura per fusione di scaffold di policaprolattone e cellule cardiache derivate da hiPSC (Video) | JoVE Journal

Generazione 3D di tessuto miocardico umano mediante scrittura di elettrofilatura per fusione di scaffold di policaprolattone e cellule cardiache derivate da hiPSC

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