Name: Interfacce elettriche e optoelettroniche bidirezionali in cuori di ratto sani e ischemici ex vivo (Video) | JoVE Journal
Uploaded: 2025-07-18T13:20:00.000+00:00
Duration: 8 min 33 s
Description: Esplora interfacce bidirezionali optoelettroniche ed elettriche in cuori di ratto ex vivo. Questo metodo consente stimolazione cardiaca, registrazione ECG e modellazione dell'infarto per la ricerca in bioelettronica.

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<table><tbody><tr><td>Headstage a 16 canali</td><td>Tecnologie Intan</td><td>Codice: C3334</td><td></td></tr><tr><td>Cloruro di 2,3,5-trifeniltetrazolio</td><td>Sigma Aldrich</td><td>T8877-5G</td><td>cloruro di trifeniltetrazolio </td></tr><tr><td>2-propanolo (IPA) </td><td>Fischer Scientific</td><td>Trasmissione A451-4</td><td></td></tr><tr><td>Laser da 635 nm, 500 mW</td><td>Bagliore laser</td><td>NA</td><td></td></tr><tr><td>Acetone</td><td>Fischer Scientific</td><td>A18-4</td><td></td></tr><tr><td>Evaporatore a fascio elettronico Angstrom Evovac</td><td>Angstrom</td><td>NA</td><td></td></tr><tr><td>Sviluppatore AZ 300MIF</td><td>Microchimica</td><td>18441123163</td><td>Sviluppatore</td></tr><tr><td>AZ nlof 2020 </td><td>Microchimica</td><td>1A002020</td><td></td></tr><tr><td>AZ NMP </td><td>Microchimica</td><td>NA</td><td>Dispositivo di rimozione del fotoresist</td></tr><tr><td>AZ40XT-11D </td><td>Microchimica</td><td>1000300</td><td>Fotoresist</td></tr><tr><td>HF tamponato</td><td>Termo Scientifico</td><td>AA44627K2</td><td></td></tr><tr><td>CaCl2.2H2O</td><td>Sigma Aldrich</td><td>(C3881)</td><td></td></tr><tr><td>Laser CO2 </td><td>Sistemi laser universali</td><td>VLS 4.60</td><td></td></tr><tr><td>Vernice conduttiva all'argento</td><td>PELCO</td><td>16062</td><td>Adesione conduttiva tra l'elettrodo e il filo di Cu di collegamento</td></tr><tr><td>Filo di rame incapsulato 0,1 mm</td><td>Amazzone</td><td>https://www.amazon.com/Enameled-Temperature-Resistance-Transformers-Inductors/dp/ B09TNM51S5/ref=asc_df_B09 TNM51S5?mcid=155de761ea4 c389ea86d45ba61a83e8d& tag =HYPROD-20& linkCode=df0& hvadid =693675076770& hvpos=& hvnetw =g& HVRand=664358781094905 4431& hvpone=& HVPTWO=& hvqmt =& hvdev=c& hvdvcmdl=& hvlocint =& hvlocphy=9021716& hvtargid= pla-1878323158943& psc=1</td><td></td></tr><tr><td>Digitalizzatore Digidata 1550</td><td>Dispositivi molecolari</td><td>NA</td><td></td></tr><tr><td>Glucosio</td><td>Sigma Aldrich</td><td>G7021</td><td></td></tr><tr><td>HBSS, senza calcio, senza magnesio, senza rosso fenolo</td><td>Termo Scientifico</td><td>14175095</td><td></td></tr><tr><td>Heidelberg MLA150 sceneggiatore diretto</td><td>Heidelberg</td><td>NA</td><td></td></tr><tr><td>eparina (1000 UI/kg)</td><td>Sagent Pharmaceuticals</td><td>1009655</td><td></td></tr><tr><td>HEPES</td><td>Sigma Aldrich</td><td>(H4034)</td><td></td></tr><tr><td>Esano </td><td>Sigma Aldrich</td><td>52750-10ML</td><td>Solvente</td></tr><tr><td>HF</td><td>Fisher Scientifico</td><td>A513-500</td><td>Grado di Tracemetal</td></tr><tr><td>HMDS Forno a vapore Prime</td><td>Sistemi di ingegneria del rendimento</td><td>NA</td><td></td></tr><tr><td>HNO3</td><td>Fisher Scientifico</td><td>A509P500</td><td>Grado di Tracemetal</td></tr><tr><td>Laminatore a caldo</td><td>Deposito uffici</td><td>L410-A</td><td>Incapsulamento multistrato </td></tr><tr><td>Sistemi Intan RHD</td><td>Tecnologie Intan</td><td>Visualizzazione del materiale C3100</td><td></td></tr><tr><td>ECG iWorx</td><td>iWorx</td><td> IA-400D</td><td></td></tr><tr><td>Sonda LVD iWorx</td><td>iWorx</td><td>BP-100</td><td></td></tr><tr><td>Preamplificatore iWorx</td><td>iWorx</td><td>C-ISO-256</td><td></td></tr><tr><td>Kcl</td><td>Sigma Aldrich</td><td>(P4504)</td><td></td></tr><tr><td>Palloncino in lattice (misura 4)</td><td>Radnoti</td><td>170404</td><td></td></tr><tr><td>MgCl2.7H2O</td><td>Sigma Aldrich</td><td>(Codice: M9272)</td><td></td></tr><tr><td>NaCl</td><td>Sigma Aldrich</td><td>(S9625)</td><td></td></tr><tr><td>Nafion 117 contenente soluzione</td><td>Sigma Aldrich</td><td>31175-20-9</td><td>Reagente di fissaggio per pattern di carbonio nanoporoso</td></tr><tr><td>NaH2PO4</td><td>Sigma Aldrich</td><td>(S0751)</td><td></td></tr><tr><td>NaOH</td><td>Sigma Aldrich</td><td>221465</td><td></td></tr><tr><td>Sensori di sicurezza</td><td>Fisher Scientifico</td><td>NC9285739</td><td></td></tr><tr><td>Plasma-Therm incisore al fluoruro al plasma accoppiato induttivamente</td><td>Plasma-Therm</td><td>NA</td><td></td></tr><tr><td>PMMA (495 A6) </td><td>Microchimica</td><td>M130006 </td><td></td></tr><tr><td>poli (dianidride-co-4,4'-ossidianilina piromellitica)</td><td>Sigma Aldrich</td><td>575801</td><td>Precursore PI</td></tr><tr><td>Poli(etilene-co-vinil acetato)</td><td>Sigma Aldrich</td><td>Codice 437247-250G</td><td>Polimero per infiltrazione</td></tr><tr><td>Poli(alcol vinilico)</td><td>Sigma Aldrich</td><td>Codice 341584-25G</td><td></td></tr><tr><td>Polietilene tereftalato (PET)</td><td>Amazzone</td><td>KS-6304-21-11</td><td>Tipo D Foglio in PET trasparente .0005" di spessore x 27" di larghezza x 10 piedi di lunghezza 1 pz</td></tr><tr><td>Potenziostato</td><td>Biologici</td><td>SP-200</td><td>Stimolazione elettrica</td></tr><tr><td>resistere al forno</td><td> Spedire</td><td>LCC1-15-5</td><td></td></tr><tr><td>Microscopia elettronica a scansione</td><td>Carl Zeiss</td><td>Smeriglio</td><td>Strumento per la caratterizzazione morfologica</td></tr><tr><td>wafer di silicio </td><td>Materiali elettronici NOVA</td><td>HS39626</td><td></td></tr><tr><td>Adesivo siliconico</td><td>WPI</td><td>KWIK-SIL-S</td><td>Incapsulamento</td></tr><tr><td>Borato di sodio</td><td>Sigma Aldrich</td><td>SX0355</td><td></td></tr><tr><td>Wafer SOI</td><td>Ultrasil</td><td>3-10541</td><td>Tipo-Orientamento:P/B(100) DT:7+/-1um DR:<. 005 Ohmcm HT:300+/-10um HR:1-30 Ohmcm Scatola:1um+/-5% UD-13998/UH-13962</td></tr><tr><td>SU-8 2002</td><td>Microchimica</td><td>SU-8 Serie 2000</td><td></td></tr><tr><td>Sviluppatore SU-8</td><td>Microchimica</td><td>SU-8 Serie 2000</td><td></td></tr><tr><td>Adesivo per tessuti umidi</td><td>3M</td><td>Codice: 1469SB</td><td></td></tr><tr><td>Carta da filtro qualitativa Whatman, Grado 1</td><td>Sigma Aldrich</td><td>WHA1001150</td><td>Substrato per la modellazione del carbonio</td></tr></tbody></table>

interfacce elettriche e optoelettroniche bidirezionali in cuori di ratto sani e ischemici ex vivo

Bidirectional Electrical and Optoelectronic Interfaces in Healthy and Ischemic Ex Vivo Rat Hearts

Interfacce elettriche e optoelettroniche bidirezionali in cuori di ratto sani e ischemici ex vivo

Electrophysiological studies are pivotal in exploring interactions between materials and cardiac biointerfaces. Recent advancements have introduced ...

bidirectional-electrical-optoelectronic-interfaces-healthy-ischemic

Research

Ricerca

JoVE Journal

Bioengineering

Bioingegneria

Questo articolo descrive una tecnica ad alta risoluzione temporale e spaziale a otticamente movimento immagine del potenziale d'azione sulla superficie del Langendorff-cuore perfuso di ratto utilizzando un colorante potenziometrica (di-8-ANEPPS).

Mappatura ottica di Langendorff-perfusi Hearts Rat

Questo articolo descrive le procedure di base per lo svolgimento di esperimenti di mappatura ottica nella Langendorff-perfuso cuore di coniglio con il sistema di imaging panoramico, e il doppio (tensione e calcio) modalità di imaging.

Multiparametrica mappatura ottica del Langendorff-perfuso Rabbit Heart

L'obiettivo di questo studio era quello di stabilire un metodo per studiare le dinamiche cardiache utilizzando un modello animale traslazionale. L'approccio sperimentale descritto incorpora l'optocardiografia a doppia emissione in combinazione con uno studio elettrofisiologico per valutare l'attività elettrica in un modello cardiaco porcina isolato e intatto.

Optocardiografia ed elettrofisiologia Studi di Ex Vivo Langendorff-perfusi

Questo protocollo descrive la procedura per sezionare e coltivare fette cardiache umane per test preclinici sui farmaci e descrive in dettaglio l'uso della mappatura ottica per la registrazione simultanea della tensione transmembrana e dei segnali di calcio intracellulare da queste fette.

Valutazione elettrofisiologia cardiaca preclinica mediante mappatura ottica a doppia tensione e calcio delle fette cardiache di organitypica umana

Questo articolo presenta un metodo efficiente per eseguire l'ischemia miocardiale e la successiva riperfusione cronica nei ratti utilizzando un approccio minimamente invasivo. Inoltre, la funzione emodinamica ventricolare sinistra dei ratti è valutata con ecocardiografia e metodi isolati del cuore di lavoro.

Metodo semi-minimo invasivo per indurre l'infarto miocardico nei ratti e la valutazione della funzione cardiaca da parte di un sistema cardiaco funzionante isolato

Questo lavoro riporta un metodo per controllare il ritmo cardiaco di cuori murini intatti di topi transgenici channelrhodopsin-2 (ChR2) utilizzando la fotostimolazione locale con un array di micro-LED e la mappatura ottica simultanea del potenziale della membrana epicardica.

Gestione avanzata del ritmo cardiaco applicando la fotostimolazione multi-sito optogenetica nei cuori murini

Questo protocollo introduce la mappatura ottica a doppio colorante di cuori di topo ottenuti da animali wild-type e knock-in affetti da tachicardia ventricolare polimorfa catecolaminergica, comprese le misurazioni elettrofisiologiche del voltaggio transmembrana e dei transitori intracellulari di Ca2+ con un'elevata risoluzione temporale e spaziale.

Mappatura ottica a doppio colorante di cuori da topi knock-in RyR2R2474S di tachicardia ventricolare polimorfa catecolaminergica

Questo protocollo illustra un metodo per la stimolazione optogenetica dei neuroni cardiaci intrinseci nei cuori di topo transgenici. L'approccio descritto è utilizzato per studiare la cinetica dell'attivazione improvvisa dei neuroni cardiaci in cuori perfusi ex vivo e le interazioni tra attività colinergica e catecolaminergica.

Attivazione optogenetica dei neuroni autonomici cardiaci intrinseci nei cuori di topo perfusi asportati

Un sistema di coltura 3D per ematopoiesi è descritta utilizzando sangue umano corda e leucemiche cellule del midollo osseo. Il metodo si basa sull'impiego di un poliuretano poroso scaffold sintetico rivestito con proteine ​​della matrice extracellulare. Questa impalcatura è adattabile per soddisfare una vasta gamma di celle.

Ex vivo Mimicry di emopoiesi normale e anormale umana

Test microtrazione Environmentally-controllato di meccanica-adattivi nanocompositi polimerici per<em&gt; Ex vivo</em&gt; Caratterizzazione

Esplora interfacce bidirezionali optoelettroniche ed elettriche in cuori di ratto ex vivo. Questo metodo consente stimolazione cardiaca, registrazione ECG e modellazione dell'infarto per la ricerca in bioelettronica.

Interfacce elettriche e optoelettroniche bidirezionali in cuori di ratto sani e ischemici ex vivo

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