Name: Microdissezione multilivello e profilazione funzionale-strutturale di rami arteriosi renali umani (Video) | JoVE Journal
Uploaded: 2025-09-05T13:00:00.000+00:00
Duration: 6 min 51 s
Description: Impara la microdissezione e la miografia a filo per valutare la funzione vascolare delle arterie renali umane. Questo metodo consente analisi funzionali precise per lo sviluppo di farmaci mirati.

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</ol>

<table><tbody><tr><td>Cloruro di acetilcolina</td><td>Sigma-Aldrich, Merck, Darmstadt, Germania</td><td>Codice: A6625</td><td></td></tr><tr><td>Piatto di coltura a fondo nero</td><td>Danese Myo Technology, Aarhus, Danimarca</td><td>300412</td><td></td></tr><tr><td>CaCl2</td><td>Sangon Biotech Co., Ltd., Shanghai, Cina</td><td>A501330</td><td></td></tr><tr><td>D-glucosio</td><td>Sangon Biotech Co., Ltd., Shanghai, Cina</td><td>A610219</td><td></td></tr><tr><td>Pinza di Dumont</td><td>Danese Myo Technology, Aarhus, Danimarca</td><td>300413</td><td></td></tr><tr><td>Forbice da dissezione Geuder</td><td>Danese Myo Technology, Aarhus, Danimarca</td><td>400431</td><td></td></tr><tr><td>Filo guida</td><td>Danese Myo Technology, Aarhus, Danimarca</td><td>400447</td><td>Diametro 40 &micro; m</td></tr><tr><td>Kcl</td><td>Sangon Biotech Co., Ltd., Shanghai, Cina</td><td>A100395</td><td></td></tr><tr><td>KH2PO4</td><td>Sangon Biotech Co., Ltd., Shanghai, Cina</td><td>A100781</td><td></td></tr><tr><td>LabChart Professional versione 8.3&nbsp;</td><td>ADInstruments, Australia</td><td>-</td><td></td></tr><tr><td>MgCl2&middot; 6H2O</td><td>Sangon Biotech Co., Ltd., Shanghai, Cina</td><td>A100288</td><td></td></tr><tr><td>Sistema multi miografo&nbsp;</td><td>Danese Myo Technology, Aarhus, Danimarca</td><td>620 milioni</td><td></td></tr><tr><td>NaCl</td><td>Sangon Biotech Co., Ltd., Shanghai, Cina</td><td>A100241</td><td></td></tr><tr><td>NaHCO3</td><td>Sangon Biotech Co., Ltd., Shanghai, Cina</td><td>A100865</td><td></td></tr><tr><td>Fenilefrina</td><td>Sigma-Aldrich, Merck, Darmstadt, Germania</td><td>P6126</td><td></td></tr></tbody></table>

microdissezione multilivello e profilazione funzionale-strutturale di rami arteriosi renali umani

Multilevel Microdissection and Functional-Structural Profiling of Human Renal Arterial Branches

Nel rene umano, l'arteria interlobare corre tra le piramidi renali lungo la vena corrispondente. Caratterizzata da una parete vascolare relativamente spessa e da un significativo rivestimento del tessuto adiposo, questa arteria richiede una dissezione particolarmente attenta. Il tessuto adiposo circostante deve essere rimosso meticolosamente per evitare l'applicazione di una forza eccessiva durante la procedura di isolamento (Figura 1C).
L'arteria arcuata è posizi...

Microdissezione multilivello e profilazione funzionale-strutturale di rami arteriosi renali umani

Renal vascular dysfunction plays a critical role in the pathogenesis of multiple clinical conditions, including acute kidney injury, renal ischemia, and ...

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Il rene adulto zebrafish è un sistema eccellente per gli studi di rigenerazione e malattie renali. Un aspetto essenziale di tale ricerca è la valutazione della struttura e funzione del nefrone. Questo protocollo descrive le diverse metodologie che possono essere implementate per valutare la composizione nefrone tubulo e per valutare il riassorbimento renale.

Analisi di Nefrone Composizione e funzione del rene Zebrafish adulti

Viene descritto l'uso della risonanza magnetica ad altissimo campo come metodo non invasivo per ottenere informazioni fenotipiche di modelli di roditori per la malattia del rene policistico e per monitorare gli interventi. Rispetto all'approccio istologico tradizionale, le immagini MRI possono essere acquisite in vivo, consentendo un follow-up longitudinale.

L&#39;uso di ultra-alto campo MRI in piccolo roditore modelli di malattia del rene policistico per<em&gt; In Vivo</em&gt; Fenotipizzazione and Drug Monitoring

Questo protocollo descrive un metodo per isolare e coltivare rudimenti metanufricani da embrioni di topo.

Dissezione e cultura del rene embrionale del mouse

Tessutale renale costrutti forniscono una soluzione per la carenza di organi e deleteri effetti della dialisi. Qui, descriviamo un protocollo a micro sezionare murini reni per isolamento di segmenti cortico-midollare. Questi segmenti sono impiantati nei costrutti senza impalcatura cellulare, formando organoids renale.

Microdissection di segmenti di tessuto renale primario e incorporazione con tecnologia romanzo costrutto senza impalcatura

Un modello murino Goldblatt modificato a 2 rene 1 clip (2K1C) è stato sviluppato utilizzando tubi in poliuretano per avviare la stenosi dell'arteria renale, inducendo un aumento dell'espressione della renina e un danno renale. Qui, descriviamo una procedura dettagliata di preparazione e posizionamento del bracciale sull'arteria renale per generare un modello murino 2K1C riproducibile e coerente.

Un modello di topo modificato a due rene una clip della regolazione della renina nella stenosi dell'arteria renale

Descriviamo un protocollo per la microdisezione laser di sottosezioni del rene umano, tra cui il glomerulus, il tubulo prossimale, l'arto ascendente spesso, il condotto di raccolta e l'interstizio. L'RNA viene quindi isolato dai compartimenti ottenuti e il sequenziamento dell'RNA viene effettuato per determinare i cambiamenti nella firma trascrittamica all'interno di ogni sottoseme.

Applicazione della microdisezione laser per scoprire la trascritomica regionale nel tessuto renale umano

Il protocollo descrive l'isolamento, la coltura e la profilazione delle cellule endoteliali dall'arteria mesenterica umana. Inoltre, viene fornito un metodo per preparare l'arteria umana per la trascrittomica spaziale. Proteomica, trascrittomica e saggi funzionali possono essere eseguiti su cellule isolate. Questo protocollo può essere riproposto per qualsiasi arteria di medie o grandi dimensioni.

Isolamento e profilazione delle cellule endoteliali arteriose mesenteriche primarie umane a livello di trascrittoma

Qui presentiamo un protocollo che utilizza la microscopia a 2 fotoni a Monaco di Baviera nei ratti Wistar Fromter con glomeruli di superficie per quantificare gli effetti dell'ostruzione ureterale prolungata sulla dinamica e sulla funzione glomerulare.

Utilizzo della microscopia a 2 fotoni per quantificare gli effetti dell'ostruzione ureterale unilaterale cronica sui processi glomerulari

Qui, presentiamo un protocollo dettagliato per l'analisi proteomica dell'intero rene, del tubulo corticale isolato e dei proteomi midollari. Lo studio confronta anche i proteomi regionali in un modello murino diabetico e in topi non diabetici.

Analisi proteomica comparativa di interi reni, midollo e tubuli corticali nella patogenesi diabetica del danno renale nei topi

Impara la microdissezione e la miografia a filo per valutare la funzione vascolare delle arterie renali umane. Questo metodo consente analisi funzionali precise per lo sviluppo di farmaci mirati.

Microdissezione multilivello e profilazione funzionale-strutturale di rami arteriosi renali umani

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