Name: 発育中および成熟 シロイヌナズ ナ種子中の胚乳細胞層の顕微鏡分析のための無傷組織の調製 (動画) | JoVE Journal
Uploaded: 2025-05-16T14:40:00.000+00:00
Duration: 6 min 28 s
Description: 学ぶ Arabidopsis thalianaの胚乳細胞層の無傷組織調製法。蛍光ライブ細胞イメージングで胚乳の動態を可視化し、種子発芽における細胞内イベントと分子メカニズムの解明に貢献します。

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<li>Shinozaki, D., Takayama, E., Kawakami, N., Yoshimoto, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Autophagy+maintains+endosperm+quality+during+seed+storage+to+preserve+germination+ability+in+Arabidopsis%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+USA%22%5BJournal%5D)">Autophagy maintains endosperm quality during seed storage to preserve germination ability in Arabidopsis.</a> Proc Natl Acad Sci USA. 121 (14), e2321612121(2024).</li>
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<li>Yoshimoto, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Processing+of+ATG8s%2C+ubiquitin-like+proteins%2C+and+their+deconjugation+by+ATG4s+are+essential+for+plant+autophagy%5BTitle%5D)+AND+%22Plant+Cell%22%5BJournal%5D)">Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy.</a> Plant Cell. 16 (11), 2967-2983 (2004).</li>
<li>Xu, G., Zhang, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mechanisms+controlling+seed+size+by+early+endosperm+development%5BTitle%5D)+AND+%22Seed+Biol%22%5BJournal%5D)">Mechanisms controlling seed size by early endosperm development.</a> Seed Biol. 2, 1(2023).</li>
</ol>

<table><tbody><tr><td>1.5 mL マイクロ遠心チューブ</td><td>Watoson Bio Lab</td><td>131-815C</td><td></td></tr><tr><td>カバーガラス (18 x 18 mm)</td><td>松波硝子工業株式会社</td><td>C218181</td><td></td></tr><tr><td>DDW</td><td></td><td></td>取<td>付</td></tr>用<tr><td>水 ろ紙 No.526 (400×400mm)</td><td>アドバンテックベトナム株式会社</td><td>02453400&nbsp;</td><td></td></tr><tr><td>元気くん せる 千陽 淀 こぷ N-150 (55L)</td><td>カタクラ&ス生協アグリコーポ</td><td></td>レーション <td>植物成長用土壌</td></tr>スライド<tr><td>ガラス (26mm×76mm)</td><td>松波硝子工業株式会社</td><td>S1215</td><td></td></tr><tr><td>Grodan AO 36 x 36 x 40 mm キューブ植物</td>成長アイリス用 <td>Grodan</td><td></td><td>ロックウール</td></tr><tr><td>&nbsp;はさみ</td><td>プレミアムプラス&nbsp;日本株式会社</td><td>FC-0212</td><td></td></tr><tr><td>ジュエラーズ鉗子、デュモン No. 5 (4 1/4 インチ)</td><td>Dumont</td><td>F6521</td><td></td></tr><tr><td>ライカ アプリケーション スイート X (LAS X)&nbsp</td> を引き裂くための鉗子;<td>Sterallis 8 Leica</td>Microsystems&nbsp 用<td>ライカ</td><td></td> ソフトウェア</tr><tr><td>;顕微鏡用浸漬油</td><td>超低自家蛍光</td>浸漬油 <td>THMOIL-10LF</td><td></td></tr><tr><td>LIOR精密鉗子 110mm&nbsp;SL-14</td><td>ケニス株式会社</td>保持用<td>鉗子KN33450438</td>&<td>nbsp;</td></tr><tr><td>ネイルホリック</td><td>コーセー</td><td></td><td>マニキュア</td></tr><tr><td>針27G 3/4(19 mm)RB&nbsp;</td><td>三沢メディカル工業株式会社</td><td></td><td></td></tr><tr><td>ニチペットエア1000uL</td>ニ<td>チリョウ</td><td>00-NAR-1000A1000</td>&microを切断するためのインジェクションニードル<td>;L マイクロピペット</td></tr><tr><td>パーフルオロデカリン</td><td>APOLLO SCIENTIFIC</td><td>PC5960</td><td></td></tr><tr><td>赤色光・遠赤色光LEDパネル</td>実装用試薬 東京<td>立垣会株式会社</td><td>10147599</td><td></td></tr><tr><td>シャッペスパン #60</td><td>株式会社富士クス</td><td></td><td>スレッド</td></tr><tr><td>SPINKOTE 潤滑剤 2 オンス</td><td>BECKMAN COULTER</td><td>306812</td><td>グリース</td></tr><tr><td>Sterallis 8</td><td>ライカ</td><td></td><td>共焦点レーザー 走査顕微鏡</td></tr><tr><td>実体顕微鏡 Stemi 305 cam W</td><td>Carl Zeiss NTS Ltd.</td><td>491903-0017-000</td><td></td></tr><tr><td>白色光 LED</td><td>パナソニック</td><td>FL40SSW/37</td><td></td></tr></tbody></table>

発育中および成熟 シロイヌナズ ナ種子中の胚乳細胞層の顕微鏡分析のための無傷組織の調製

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

図1に示すプロトコルを使用して、胚乳サンプルは、14 DAFでシリックから収穫された発育中の種子から調製されました(この段階では、精巣はまだ緑色です)。広い領域にわたる多数の胚乳細胞とその細胞内構造が観察されました(図3A)。この実験では、C末端でGFPと融合したPHYBを発現するシーズ(PHYB-GFP)を使用しました。PHYBは赤色光による活性化により核に移動し、光体(PB)として知られるPHYB陽性のスペックルを形成し、これが種子の発芽に関与することはよく知られている4,20,21,22。サンプルは、22°Cの連続白色光条件下で栽培された植物から収穫されました。 図3Bに示すように、核内のPBは胚乳細...

In Arabidopsis seeds, the endosperm, a single layer of living cells located between the embryo and the testa, plays a critical role in regulating seed ...

preparation-intact-tissue-for-microscopic-analysis-endosperm-cell

Research

JoVE Journal

Biology

生物学

刷り込みは、植物と哺乳類の生殖の現象です。...

のインプリント遺伝子のDNAメチル化の定量シロイヌナズナ胚乳

我々は、気孔の分化を文書化、開発の数日間にわたって表皮の共焦点イメージングのための植物の子葉を固定化するためにチャンバースライドやメディアを使用してプロトコルを記述します。フルオロフォアタグ付きタンパク質は、細胞分裂や細胞型分化の過程でそれらの可能な役割の理解を深め、表現と細胞内局在によって動的に追跡することができます。...

の長期、高分解能共焦点タイムラプスイメージング<em&gt;シロイヌナズナ子葉</em発芽過程&gt;表皮

我々は、より開発の初期段階で胚を単離するための効率的で簡単な方法を報告する...

から初期段階の胚の効率的かつ迅速な単離<em&gt;シロイヌナズナ</em&gt;種子

私たちは、 シロイヌナズナの種子から種皮寝具アッセイ（SCBA）を構築する手順を紹介します。 SCBAは、遺伝的およびin vitroで探索する強力なツールであることが示されたか休眠種子中の光の合図に応答して、胚乳を制御し種子の発芽。 SCBAは胚乳が胚の成長に影響を与えることが疑われるあらゆる状況下で適用可能な原則です。...

遺伝的に探索するために種皮寝具アッセイ<em&gt;体外</em&gt;どのように胚乳コントロール種子発芽<em&gt;シロイヌナズナ</em

私たちは、免疫染色、蛍光in situハイブリダイゼーション 、ホールマウントシロイヌナズナ胚珠の量的、高分解能イメージングに続いてDNA染色のためにここに効率的で信頼性の高いプロトコルを提供する。この方法は、正常なクロマチン修飾および核構造を分析するために使用した。...

ホールマウント胚珠内でのクロマチン修飾や核構造の定量的、単一細胞分析のための効率的な方法<em&gt;シロイヌナズナ</em

結晶性セルロースは、植物の細胞壁の重要な構成要素です。しかし、細胞分解能での定量化は技術的に困難です。...

結晶セルロース蓄積中の高解像度の定量化<em&gt;シロイヌナズナ</em&gt;ルーツは、組織特異的な細胞壁の変更を監視します

このプロトコルでは、顕微鏡下で胚パターン形成の検査続く胚珠クリアランスを介してシロイヌナズナの胚発生を観察するためのメソッドについて説明します。...

シロイヌナズナの胚発生の特性評価法

このプロトコルでは、シロイヌナズナの花と...

全体マウント クリアとシロイヌナズナの花器官および Siliques の染色

この技術は、種子の異なる領域における細胞、デンプン顆粒およびタンパク質体の観察と分析のための全種サイズの樹脂セクションの迅速かつ簡単な調製を可能にする。...

全種サイズ樹脂断面の簡易ドライ断面化方法とその応用

本稿では,シロイヌナズナの花粉管誘導・受容観察のためのセミインビトロ(SIV)法の改良により,胚珠の受容性を高める方法について述べる.ハイスループットSIV兼セプタム法は、配偶体マーカーラインおよび遺伝的にコード化されたバイオセンサーと組み合わせて、受精の動的プロセスを監視できます。...

セミインビトロ中隔法によるシロイヌナズナの花粉管受容と二重受精のライブイメージング

学ぶ Arabidopsis thalianaの胚乳細胞層の無傷組織調製法。蛍光ライブ細胞イメージングで胚乳の動態を可視化し、種子発芽における細胞内イベントと分子メカニズムの解明に貢献します。

発育中および成熟シロイヌナズナ種子中の胚乳細胞層の顕微鏡分析のための無傷組織の調製

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発育中および成熟 シロイヌナズ ナ種子中の胚乳細胞層の顕微鏡分析のための無傷組織の調製

さらに動画を探す

発育中および成熟シロイヌナズナ種子中の胚乳細胞層の顕微鏡分析のための無傷組織の調製