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Live-cell imaging en kwantitatieve analyse van de embryonale epitheelcellen in Xenopus laevis
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Live-cell imaging en kwantitatieve analyse van de embryonale epitheelcellen in Xenopus laevis

Article DOI: 10.3791/1949-v • 06:52 min • May 23rd, 2010
May 23rd, 2010 •
1, 1,2
1Bioengineering, University of Pittsburgh, 2Developmental Biology, University of Pittsburgh

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Xenopus embryonale epitheel is een ideaal modelsysteem om cel gedrag, zoals polariteit ontwikkeling en vorm veranderen tijdens epitheliale morfogenese bestuderen. Traditionele histologie van vaste monsters wordt in toenemende mate aangevuld door live-cell imaging confocale. Hier laten we zien methoden om de kikker weefsel te isoleren en te visualiseren live-epitheliale cellen en hun cytoskelet met behulp van live-cell confocale microscopie.

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Developmental Biology epitheelcellen kikkers Xenopus laevis epitheel meercellige hoge-resolutie confocale microscopie dier cap explantatie embryo's
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