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PAR-Clip - een methode om Transcriptome-breed de bindingsplaatsen van RNA-bindende eiwitten te identificeren
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PAR-Clip - een methode om Transcriptome-breed de bindingsplaatsen van RNA-bindende eiwitten te identificeren

Article DOI: 10.3791/2034-v • 12:24 min • July 2nd, 2010
July 2nd, 2010 •
1, 2, 3, 3, 4, 4, 1, 1, 2, 2, 1, 1, 5, 3, 1
1Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, 2Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, 3Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 4Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 5Genomics Resource Center, Rockefeller University

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RNA transcripten zijn onderworpen aan een uitgebreide posttranscriptionele regulering die wordt bemiddeld door een veelheid van trans-werkende RNA-bindende eiwitten (RBPs). Hier presenteren wij een generaliseerbaar methode om te identificeren precies en op een transcriptoom schaal van de RNA-bindingsplaatsen van RBPs.

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Cellular Biology UV crosslinking RNA bindende eiwitten RNA-bindend motief 4-thiouridine 6-thioguanosine
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