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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Article DOI: 10.3791/2034-v • 12:24 min • July 2nd, 2010
July 2nd, 2010 •
1, 2, 3, 3, 4, 4, 1, 1, 2, 2, 1, 1, 5, 3, 1
1Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, 2Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, 3Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 4Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 5Genomics Resource Center, Rockefeller University

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요약
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RNA transcripts are subject to extensive posttranscriptional regulation that is mediated by a multitude of trans-acting RNA-binding proteins (RBPs). Here we present a generalizable method to identify precisely and on a transcriptome-wide scale the RNA binding sites of RBPs.

Tags

PAR-CliP Transcriptome-wide Binding Sites RNA-binding Proteins Post-transcriptional Gene Regulation RNA Transcripts RNA-RBP Interactions MicroRNA-containing Ribonucleoprotein Complexes Cell-type Dependently Interplay High Resolution Maps In Vivo Protein-RNA Interactions Genetic Approaches Biochemical Approaches Computational Approaches Microarray Profiling RIP-Chip Kinetically Stable Interactions RBP Recognition Element (RRE) Direct RBP Target Site Information UV Crosslinking Immunoprecipitation CLIP UV 254 Nm RNA-protein Crosslinking
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