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Analyse van de pluripotente stamcellen door gebruik te maken van vriescoupes Embryoid Bodies

Article DOI: 10.3791/2344-v • 06:55 min • December 8th, 2010
December 8th, 2010 •
*1, *1, *1, 1, 1
1Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Brazil
* These authors contributed equally

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Pluripotente stamcellen in suspensie te groeien differentiëren in embryoid organen (EBS). Hier laten we zien hoe een hoge kwaliteit EB vriescoupes handig voor het bestuderen van cellulaire en moleculaire aspecten van de embryogenese te verkrijgen, met behoud van hun organisatie als aggregaten.

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Developmental Biology Embryonale stamcellen embryoid lichaam vriescoupes immunochytochemistry H9
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