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Étiquetage des cellules Lineage Zebrafish avec laser Uncagable dextran fluorescéine

Article DOI: 10.3791/2672-v • 07:36 min • April 28th, 2011
April 28th, 2011 •
1, 2, 2, 1
1Department of Biological Sciences, Vanderbilt University, 2Department of Chemical and Systems Biology, Stanford University

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Ce protocole définit un moyen d'étiqueter et de tracer le destin de petits groupes de cellules du poisson zèbre en utilisant UV-uncaging de fluorescéine en cage, suivi par l'ensemble de montage immunomarquage pour amplifier le signal de la fluorescéine Uncaged.

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Biologie du Développement numéro 50 le poisson-zèbre l'étiquetage lignée cage fluorescéine transgène
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