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Expressionsanalyse von Säugetier-Linker-Histon-Subtypen

Article DOI: 10.3791/3577-v • 14:40 min • March 19th, 2012
March 19th, 2012 •
1, 1, 1, 1
1School of Biology and the Parker H. Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology

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Wir beschreiben eine Reihe von Tests zur Expression von H1 Linker Histonen zu analysieren. mRNA einzelner Gene H1 werden quantitativ durch zufällige Primer basieren reverse Transkription durch real-time PCR gefolgt gemessen, während Protein Quantifizierung der H1-Histone durch HPLC-Analyse erreicht wird.

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Genetik H1 Linker Histonen Histon H1-Subtypen Chromatin RT-PCR HPLC Genexpression
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