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Visualisierung von Cortex Organisation und Dynamik in Mikroorganismen, mit Total Internal Reflection Fluorescence Microscopy

Article DOI: 10.3791/3982-v • 14:15 min • May 1st, 2012
May 1st, 2012 •
1, 1, 1,2, 1
1AG Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, 2Helmholtz Zentrum München

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Total Internal Reflection Fluorescence (TIRF)-Mikroskopie ist eine leistungsfähige Methode, um Strukturen in der Nähe der Zelloberfläche bei hohem Kontrast und zeitlicher Auflösung zu beobachten. Wir zeigen, wie TIRF eingesetzt werden können, um Protein-Dynamik in der Rinde der Zellwand-geschlossenen Zellen von Bakterien und Pilzen zu studieren.

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Bioengineering TIRF Imaging- Hefe Bakterien Mikroskopie Zellcortex
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