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Journal / Bioengineering / Cell Mønster på Photolithographically Definert…
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Bioengineering

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JoVE Journal Bioengineering
Cell Mønster på Photolithographically Definert Parylene-C
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Cell Mønster på Photolithographically Definert Parylene-C: SiO

Article DOI: 10.3791/50929-v • 07:19 min • March 7th, 2014
March 7th, 2014 •
1, 2, 3, 1, 3
1Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, 2Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, 3School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh

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Denne protokollen beskriver en microfabrication-kompatibel metode for celle mønster på SiO 2. En forhåndsdefinert parylene-C design er photolithographically trykt på SiO 2 wafere. Etter inkubasjon med serum (eller annen aktivisering løsning) celler holder seg spesifikt til (og vokse i henhold til konformitet) underliggende parylene-C, mens blir frastøtt av SiO 2 regioner.

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Bioteknologi reseptorer Cell Surface Polymers celle adhesjon Biomedical og Dental Materials parylene-C silisiumdioksid fotolitografi celle adhesjon Cell Mønster
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