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JoVE Journal Chemistry
Bestimmung der eisbindenden Ebenen von Frostschutzproteinen durch Fluoreszenzbasierte Eisebene Affinität
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Bestimmung der eisbindenden Ebenen von Frostschutzproteinen durch Fluoreszenzbasierte Eisebene Affinität

Article DOI: 10.3791/51185-v • 08:46 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 3, 4, 1
1Department of Biomedical and Molecular Sciences, Queen's University, 2National Institute of Neurological Disorders and Stroke, Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition, The Hebrew University of Jerusalem

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Frostschutzproteine (AFPs) binden an bestimmte Eisebenen, um das Eiswachstum zu verhindern oder zu verlangsamen. Die Fluoreszenzbasierte Eisebene (FIPA)-Analyse ist eine Modifikation der ursprünglichen Eisätzmethode zur Bestimmung von AFP-gebundenen Eisebenen. AFPs werden fluoreszierend beschriftet, in makroskopische Einzeleiskristalle integriert und unter UV-Licht visualisiert.

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Chemie Ausgabe 83 Materialien Biowissenschaften Optik Frostschutzproteine Eisadsorption Fluoreszierende Etikettierung Eisgitterebenen Eisbindende Proteine Eineiskristall
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