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Het bepalen van de IJsbindende Vlakken van Antivries eiwitten door Fluorescentie-gebaseerde Ice Plane Affiniteit

Article DOI: 10.3791/51185-v • 08:46 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 3, 4, 1
1Department of Biomedical and Molecular Sciences, Queen's University, 2National Institute of Neurological Disorders and Stroke, Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition, The Hebrew University of Jerusalem

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Antivrieseiwitten (ACP's) binden zich aan specifieke ijsvlakken om ijsgroei te voorkomen of te vertragen. Fluorescentie-gebaseerde ijsvlakaffiniteitsanalyse (FIPA) is een wijziging van de oorspronkelijke ijsetsmethode voor de bepaling van AFP-gebonden ijsvlakken. AFP's zijn fluorescerend gelabeld, verwerkt in macroscopische enkelvoudige ijskristallen en gevisualiseerd onder UV-licht.

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Chemie Materialen Life Sciences Optica antivries eiwitten IJs adsorptie Fluorescerende etikettering IJsroostervlakken ijsbindende eiwitten Enkel ijskristal
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