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Journal / Chemistry / 蛍光ベースの氷面アフィニティーによる不凍タンパク質の氷結合平面の決定…
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JoVE Journal Chemistry
蛍光ベースの氷面アフィニティーによる不凍タンパク質の氷結合平面の決定
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蛍光ベースの氷面アフィニティーによる不凍タンパク質の氷結合平面の決定

Article DOI: 10.3791/51185-v • 08:46 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 3, 4, 1
1Department of Biomedical and Molecular Sciences, Queen's University, 2National Institute of Neurological Disorders and Stroke, Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition, The Hebrew University of Jerusalem

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不凍タンパク質(AFP)は、氷の特定の平面に結合して、氷の成長を防ぐか、または遅くします。蛍光ベースの氷面親和性(FIPA)分析は、AFP結合した氷面の決定のためのオリジナルの氷エッチング法の改変である。AFPは蛍光標識され、巨視的な単氷結晶に組み込まれ、UV光下で可視化されます。

Tags

化学 問題 83 材料 生命科学 光学 不凍タンパク質 氷吸着 蛍光標識 氷格子 氷結合タンパク質 単氷結晶
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