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Bottom-up-und Shotgun Proteomics, um eine umfassende Cochlear Proteome Identifizieren

Article DOI: 10.3791/51186-v • 14:24 min • March 7th, 2014
March 7th, 2014 •
1, 1
1Department of Otolaryngology, Morsani College of Medicine, University of South Florida

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Proteomanalyse des Cochlea-sensorischen Epithel kann eine Herausforderung aufgrund seiner geringen Größe und weil Membranproteine ​​sind schwer zu isolieren und zu identifizieren. Sowohl Membran und lösliche Proteine ​​können durch die Kombination mehrerer Herstellungsverfahren und Trennverfahren mit hochauflösenden Massenspektrometrie identifiziert werden.

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Biochemie Cochlear Chromatographie LC-MS/MS Massenspektrometrie Proteomics sensorischen Epithel
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