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JoVE Journal Biology
Anvendelse af retinsyre hentes osteocytter kulturer fra Primary Mouse Osteoblaster
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Anvendelse af retinsyre hentes osteocytter kulturer fra Primary Mouse Osteoblaster

Article DOI: 10.3791/51465-v • 07:13 min • May 13th, 2014
May 13th, 2014 •
1, 2, 1, 1, 1, 3, 1, 1, 1, 1
1Renal Research Laboratory, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 2Department of Nephrology, Dialysis and Renal Transplant, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 3Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences, University of Trieste

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Behandling af primære muse osteoblaster med retinsyre producerer en homogen population af forgrenede celler, der bærer morfologiske og molekylære egenskaber af osteocytter. Fremgangsmåden overvinder vanskeligheden ved at opnå og bevare primære osteocytter i kultur og kan være fordelagtigt at undersøge celler afledt fra transgene modeller.

Tags

Cellular Biology cellebiologi cellekultur knogler retinsyre primære osteoblaster osteocytter celledifferentiering mus calvaria sclerostin fibroblastvækstfaktor 23 mikroskopi immunfarvning
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