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Kwantificeren Single microvessel permeabiliteit in Geïsoleerde-Blood geperfuseerde Rat Lung Voorbereiding

Article DOI: 10.3791/51552-v • 07:22 min • June 30th, 2014
June 30th, 2014 •
1, 1,2
1Department of Physiology, The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering, The University of Tennessee Health Science Center

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De geïsoleerde bloed geperfundeerd long bereiding maakt het mogelijk om microvaatje netwerken visualiseren op het longoppervlak. Hier beschrijven we een aanpak om de permeabiliteit van enkele bloedvaten in geïsoleerde longen te kwantificeren met behulp van real-time fluorescentie beeldvorming.

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Anatomie acute longschade haarvaten, FITC dextran fluorescentie microscopie beeldvorming Metamorph venule
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