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JoVE Journal Biology
A cultura
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
Journal (Biology)
Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids
Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
Journal (Biology)
Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana
 
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A cultura

Article DOI: 10.3791/51796-v • 08:26 min • August 2nd, 2014
August 2nd, 2014 •
1, 1, 1, 1,2
1Department of Animal and Avian Sciences, University of Maryland, 2Department of Cell Biology and Molecular Genetics, University of Maryland

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C. elegans é normalmente cultivadas em placas de agar sólido ou em meio líquido semeadas com E. coli. Para evitar subprodutos bacterianos de confundir os estudos toxicológicos e nutricionais, utilizamos um meio líquido axênico, CEHR, para crescer e sincronizar um grande número de vermes para uma gama de aplicações a jusante.

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Molecular Biology Edição 90, mídia axênicas transgênicos micropartículas bombardeio heme nutrição
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