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Measuring Fluxes of Mineral Nutrients and Toxicants in Plants with Radioactive Tracers

Article DOI: 10.3791/51877-v • 13:14 min • August 22nd, 2014
August 22nd, 2014 •
1, 1, 1, 1
1Department of Biological Sciences, University of Toronto

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In planta measurement of nutrient and toxicant fluxes is essential to the study of plant nutrition and toxicity. Here, we cover radiotracer protocols for influx and efflux determination in intact plant roots, using potassium (K+) and ammonia/ammonium (NH3/NH4+) fluxes as examples. Advantages and limitations of such techniques are discussed.

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Measuring Fluxes Mineral Nutrients Toxicants Plants Radioactive Tracers Unidirectional Influx Efflux Net Fluxes Nutrition Toxicology Radioisotope Tracing Plants And Environments Radiotracer Protocols Transport Systems Specific Mineral Nutrients Toxicants Compartmentation Turnover Rates Subcellular Mineral Pools Metabolite Pools Direct Influx (DI) Compartmental Analysis By Tracer Efflux (CATE) Potassium (K+) Ammonia/ammonium (NH3/NH4+) Intact Seedlings Barley (Hordeum Vulgare L.) Experimental Systems Advantages Limitations
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