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Langsiktig Time Lapse Imaging av Mouse Cochlea eksplantater

Article DOI: 10.3791/52101-v • 10:43 min • November 2nd, 2014
November 2nd, 2014 •
1, 1,2,3
1Biological Sciences Platform, Sunnybrook Research Institute, 2Department of Otolaryngology - Head and Neck Surgery, University of Toronto, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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Live-avbildning av den embryonale pattedyr cochlea er utfordrende fordi de utviklingsprosesser for hånden operere på en tidsmessig gradient over ti dager. Her presenterer vi en metode for dyrking og deretter bildebehandling embryonale cochlea explant vev tatt fra en fluorescerende reporter musen over fem dager.

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Bioteknologi Live-imaging tidsforløp cochlea øre reporter mus utvikling inkubator mikroskop Sox2
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