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JoVE Journal
Chemistry

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利用停流荧光和标记的核苷酸来分析驱动蛋白的ATP周转周期
 
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利用停流荧光和标记的核苷酸来分析驱动蛋白的ATP周转周期

Article DOI: 10.3791/52142-v 07:25 min October 17th, 2014
October 17th, 2014

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驱动蛋白的特点是核苷酸依赖的互动与微管:ATP周转连接到微管相互作用的循环周期。在这里,我们描述的协议分析个别碱基的转换中驱动蛋白的ATP周转周期使用荧光标记的核苷酸和停流荧光动力学。

Tags

化学,第92期,驱动蛋白,ATP的营业额,mantATP,mantADP,停流荧光,微管,酶动力学,核苷酸
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