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HeLa Baserat Cell yttrandefriheten System för uttryck av

Article DOI: 10.3791/52772-v • 09:03 min • June 10th, 2015
June 10th, 2015 •
1, 1
1Department of Biological, Geological, and Environmental Sciences, Cleveland State University

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Uttryck av malaria proteiner i cellbaserade system är fortsatt utmanande. Vi visar två steg och ett steg IVT (in vitro-translation) cellfritt expressionssystem för att uttrycka malaria rekombinanta rhoptry proteiner från HeLa-celler. Vi använder ett Ni-hartsaffinitetsbaserade reningssystem för att rena rhoptry proteiner.

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Biokemi Cell gratis transkription in vitro-translation HeLa-cell yttrandefriheten rhoptry proteiner däggdjurscellfria expressionssystem, Pro Bond affinitetsrening
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