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Basierend HeLa Zellfreie Expressionssysteme zur Expression von

Article DOI: 10.3791/52772-v • 09:03 min • June 10th, 2015
June 10th, 2015 •
1, 1
1Department of Biological, Geological, and Environmental Sciences, Cleveland State University

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Expression des Malaria-Proteinen in zellbasierten Systemen bleibt eine Herausforderung. Wir zeigen zwei Schritt und einen Schritt IVT (in vitro-Translation) zellfreie Expressionssysteme zur Expression von rekombinanten Malaria-rhoptry Proteine ​​aus HeLa-Zellen. Wir verwenden eine Ni-Harz Affinität Kläranlage, die rhoptry Proteine ​​zu reinigen.

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Biochemie Heft 100 Zellfreie in vitro Transkriptions-Translations HeLa-Zell-freien Ausdruck rhoptry Proteine Säugetierzellexpressionssystem kostenlos, Pro Bond Affinitätsreinigung
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