Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Sarah J. Smith; Eric J. Horstick; Ann E. Davidson; James Dowling

doi:10.3791/53183

Journal

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발생학

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에반스 블루 염료와 Zebrafish의 애벌레 골격 근육 무결성 분석

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

JoVE 신문
발생학

This content is Free Access.

JoVE 신문 발생학

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

에반스 블루 염료와 Zebrafish의 애벌레 골격 근육 무결성 분석

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9,080 Views

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07:34 min

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November 30, 2015

DOI:

10.3791/53183-v

DOI:

10.3791/53183-v

•

07:34 min

November 30, 2015

•

11 Views

Sarah J. Smith*^1,2, Eric J. Horstick*^3,4, Ann E. Davidson^1,2, James Dowling^1,2,4

¹Program in Genetics & Genome Biology,The Hospital for Sick Children, ²Department of Molecular Genetics,The University of Toronto, ³Program in Genomics of Differentiation,Eunice Kennedy Shriver National Institute of Child Health and Human Development, ⁴Departments of Pediatrics and Neurology,University of Michigan

Summary

In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae. This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.